Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro
| Autor: | Lei Yang, Zhou-Shan Tao, Zijian Shen, Lingling Zhou, Zhongjie Xie, She-Ji Weng, Bing-Li Bai, Zong-Yi Wu, Viraj Boodhun, Hang Li, Qiang Zhou, Jiahao Tang, De-Yi Yan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases medicine.medical_specialty Ovariectomy Osteocalcin H&E stain Gene Expression Osteoclasts Core Binding Factor Alpha 1 Subunit Cinnamaldehyde Collagen Type I Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Osteoclast Osteogenesis Internal medicine medicine Animals Acrolein Cells Cultured Pharmacology Osteoblasts biology Dose-Response Relationship Drug lcsh:RM1-950 Osteoblast Cell Differentiation Alkaline Phosphatase Stimulation Chemical RUNX2 Collagen Type I alpha 1 Chain 030104 developmental biology medicine.anatomical_structure Endocrinology lcsh:Therapeutics. Pharmacology chemistry 030220 oncology & carcinogenesis Depression Chemical Ovariectomized rat biology.protein Molecular Medicine Alkaline phosphatase Osteoporosis Female Phytotherapy |
| Zdroj: | Journal of Pharmacological Sciences, Vol 138, Iss 1, Pp 63-70 (2018) |
| ISSN: | 1347-8648 |
| Popis: | To explore the effect of cinnamaldehyde on the distal femur in ovariectomized rats and its influence on osteoblast in vitro. Female Sprague–Dawley rats which underwent either bilateral ovariectomy or sham operation were divided into five groups randomly: group OVX (OVX, N = 10) and group sham (SHAM, N = 10) received normal saline (NS) by gavage at a dose of 50 ml/kg·d; group low dose, group middle dose and group high dose received cinnamaldehyde by gavage at a dose of 25 mg/kg·d (OLD, N = 10), 50 mg/kg·d (OMD, N = 10), and 75 mg/kg·d (OHD, N = 10) respectively. Distal femurs were harvested for hematoxylin and eosin (HE) staining, micro-ct scanning and immunohistochemical analysis. Murine mesenchymal stem cells were cultured and dealt with the presence of either cinnamaldehyde at a dose of 15ug/ml (OLD), 30ug/ml (OMD), 60ug/ml (OHD) or vehicle. ALP staining and western blot were performed to observe the influence of cinnamaldehyde on the differentiation of osteoblast. HE and micro-ct results indicated that osteogenesis were promoted with the treatment of cinnamaldehyde. Immunohistochemical results showed that cinnamaldehyde increased the number of osteoblast and decreased the number of osteoclast. In vitro studies indicated that cinnamaldehyde promoted expression of alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN) and collagen type Iɑ1 (COL1ɑ1). The treatment effect behaved as dose-dependently. Thus, cinnamaldehyde inhibits osteoclastogenesis and promotes osteoblastogenesis, and may plays an important role in the treatment of osteoporosis clinically. Keywords: Cinnamaldehyde, Anti-Osteoporosis, Bone morphology, Osteoblast, Osteoclast |
| Databáze: | OpenAIRE |
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