Aquaglyceroporins 3 and 7 in bull spermatozoa: identification, localisation and their relationship with sperm cryotolerance
| Autor: | Joan E. Rodríguez-Gil, C. O. Hidalgo, Roser Morató, Marc Yeste, Noelia Prieto-Martínez, Sergi Bonet, R. Muiño |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (Espanya) |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Aquaporines Acrosome reaction Reproductive technology Cryopreservation 0302 clinical medicine Endocrinology reproductive and urinary physiology Fertilisation Principal Component Analysis 030219 obstetrics & reproductive medicine Microscopy Confocal medicine.diagnostic_test Bestiar boví -- Espermatozoides -- Criopreservació Anatomy Immunohistochemistry Spermatozoa Protein Transport Sperm Midpiece Acrosome Immunocytochemistry Aquaglyceroporins Biotechnology Animals Inbred Strains endocrine system Cattle -- Spermatozoa -- Cryopreservation Cell Survival Bestiar boví -- Espermatozoides -- Investigació Semen analysis Biology Aquaporins Andrology 03 medical and health sciences Genetics medicine Animals Molecular Biology Immunocitoquímica Cattle -- Spermatozoa -- Research Aquaporin 3 urogenital system Acrosome Reaction Reproducibility of Results Sperm Molecular Weight Semen Analysis 030104 developmental biology Reproductive Medicine Linear Models Animal Science and Zoology Cattle Spermatogenesis Biomarkers Developmental Biology Semen Preservation |
| Zdroj: | © Reproduction, Fertility and Development, 2017, vol. 29, núm. 6, p. 1249-1259 Articles publicats (D-B) DUGiDocs – Universitat de Girona instname |
| ISSN: | 1031-3613 |
| Popis: | The present study aimed to determine the localisation of aquaglyceroporins 3 (AQP3) and 7 (AQP7) in bull spermatozoa and their relationship with the sperm cell's resilience to withstand cryopreservation (i.e. cryotolerance). A total of 18 bull ejaculates were cryopreserved and their sperm quality analysed before and after freeze-thawing. The presence and localisation of AQP3 and AQP7 was determined through immunoblotting and immunocytochemistry. AQP3 was found in the mid-piece and AQP7 in the mid-piece and post-acrosomal region of bull spermatozoa. Immunoblotting showed specific signal bands at 30 and 60kDa for AQP3 and at 25kDa for AQP7. Neither the relative abundance of AQP3 and AQP7 nor their localisation patterns was altered by cryopreservation but individual differences between bull ejaculates were found in immunoblots. In order to determine whether these individual differences were related to sperm cryotolerance, bull ejaculates were classified as having good (GFE) or poor freezability (PFE) on the basis of their sperm quality after thawing. While the relative abundance of AQP3 before cryopreservation did not differ between ejaculates with GFE and PFE, the abundance of AQP7 was higher in GFE than in PFE ejaculates. This finding was further confirmed through principal component and linear regression analyses. In conclusion, the relative abundance of AQP7 in fresh semen may be used as a marker to predict bull sperm cryotolerance The authors acknowledge support from the Regional Government of Catalonia (Generalitat de Catalunya, Spain; Grant code: 2009-SGR-745) and the Ministry of Economy and Competitiveness, Spain (Grant code: AGL2013–47798-P). N. P.-M. was supported by a PhD Studentship funded by the Regional Government of Catalonia (Generalitat de Catalunya). R.M. was supported by a Postdoctoral Research Fellowship (‘Juan de la Cierva’ Scheme; Ministry of Economy and Competitiveness, Spain). M. Y. is funded by the European Commission, FP7-People Program, Marie Curie-IEF (Grant Number: 626061). |
| Databáze: | OpenAIRE |
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