Cyclic adenosine 3',5'-monophosphate regulation of membrane energetics in Escherichia coli
| Autor: | W J Dobrogosz, S E Dills |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Adenylate kinase
Ascorbic Acid Biology Microbiology Cyclase Receptors Cyclic AMP Electron Transport chemistry.chemical_compound Cyclic nucleotide Adenosine Triphosphate Oxygen Consumption Bacterial Proteins Flavins Cyclic AMP Escherichia coli medicine NADH NADPH Oxidoreductases Molecular Biology Flavin adenine dinucleotide Chemiosmosis Cell Membrane Quinones Wild type Hydrogen-Ion Concentration Adenosine chemistry Biochemistry Mutation Flavin-Adenine Dinucleotide Lactates Protons Oxidoreductases Adenosine triphosphate Research Article Adenylyl Cyclases medicine.drug |
| Zdroj: | Journal of Bacteriology. 131:854-865 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.131.3.854-865.1977 |
| Popis: | Mutants of Escherichia coli K-12 lacking functional adenylate cyclase (cya) or the cyclic adenosine 3',5'-monophosphate (cAMP) receptor protein (crp) were compared with their wild type to evaluate the role played by the cAMP-cAMP receptor protein complex in regulating this organism's membrane-associated bioenergetic functions. Both mutants were found to be equally defective in carrying out various electron transport activities. In particular, their capacity for synthesizing a functional oxygen-linked transhydrogenase system was totally repressed, and their content of flavin adenine dinucleotide was reduced by approximately 85%. In addition, it was found that the mutant strains had a decreased ability to generate a protonmotive force and to use this chemiosmotic force to generate adenosine 5'-triphosphate. All these membrane-associated dysfunctions were completely restored to the wild-type state when the cya cells were grown in the presence of exogenous cAMP. As would be expected if these controls were operating at the transcriptional level, the crp cells retained the mutant character even when grown in the presence of this cyclic nucleotide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|