Molecular analysis of the Septoria nodorum beta-tubulin gene and characterization of a benomyl-resistance mutation
| Autor: | Cooley Rn, Caten Ce |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Sequence analysis
Genes Fungal Molecular Sequence Data Biology Regulatory Sequences Nucleic Acid Sense Codon Tubulin Genetics Coding region Amino Acid Sequence Molecular Biology Gene Base Composition Base Sequence Sequence Homology Amino Acid Nucleic acid sequence Intron Chromosome Mapping Drug Resistance Microbial Sequence Analysis DNA Molecular biology Stop codon Introns Mutation Benomyl Mitosporic Fungi GC-content |
| Zdroj: | Moleculargeneral genetics : MGG. 237(1-2) |
| ISSN: | 0026-8925 |
| Popis: | The complete nucleotide sequence of a benomyl-resistant allele of the Septoria nodorum beta-tubulin gene (tubAR) has been determined including 745 and 1024 nucleotides 5' and 3' to the tubAR coding region, respectively. tubAR encodes a 447 amino acid polypeptide which shows a high degree of homology with other fungal beta-tubulins. The gene contains three introns at codons 4, 12 and 53, uses 48 of the possible 61 sense codons and has a GC content of 59.1% in its coding region. S1 nuclease mapping has identified two transcriptional start sites 80 bp and 83 bp upstream of the translation start, and a transcriptional termination site 192 bp downstream of the stop codon. The two transcriptional start sites lie just 8 bp and 5 bp downstream of a CT motif consisting of 18 pyrimidine nucleotides interrupted by a single adenine. The wild-type allele tubA+ has been cloned using the polymerase chain reaction and the mutation producing the benomyl-resistant phenotype of tubAR mapped to a C to T transition at the first position of codon 6, resulting in a histidine to tyrosine amino acid substitution. |
| Databáze: | OpenAIRE |
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