Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats

Autor:	Juan C. Simes, Soledad Dávila, José A. Palma, David Cremonezzi, María Belén Vignola, V Campana
Rok vydání:	2012
Předmět:	Pemf medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Pulsed Electromagnetic Field Therapy Magnetic Field Therapy Biophysics Medicine (miscellaneous) Nitric Oxide Fibrinogen medicine.disease_cause Nitric oxide Superoxide dismutase chemistry.chemical_compound Muscular Diseases Internal medicine medicine Animals Rats Wistar Myopathy Inflammation biology Superoxide Dismutase business.industry Experimental Myopathy Patología General Medicine Inflammatory biomarkers Rats Carrageenan Medicina Básica Oxidative Stress Endocrinology chemistry Magnetotherapy biology.protein Inflammatory Biomarkers Citrulline Female medicine.symptom business Pulsed electromagnetic field therapy Biomarkers Oxidative stress medicine.drug
Zdroj:	Electromagnetic Biology and Medicine. 31:320-332
ISSN:	1536-8386 1536-8378
Popis:	The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies. Fil: Vignola, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Dávila, Soledad. Universidad Nacional de La Rioja; Argentina Fil: Cremonezzi, David. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba; Argentina Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina Fil: Palma, José A.. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina Fil: Campana, Vilma. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Universidad Nacional de La Rioja; Argentina
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::202d2a565af89bb82ee7b928ac252586 https://doi.org/10.3109/15368378.2011.641706 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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