Forward Genetic Screening for the Improved Production of Fermentable Sugars from Plant Biomass
| Autor: | Xurong Tang, Danielle Vidaurre, Isaac Shim, Peter McCourt, George Stamatiou, Wolfgang Moeder, Dario Bonetta |
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| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Science Arabidopsis Carbohydrates Biomass Plant Science Genes Plant Plant Genetics 01 natural sciences 7. Clean energy Sugar acids 03 medical and health sciences Auxin Genetic model Botany Cluster Analysis Genetic Testing Sugar Biology 030304 developmental biology 2. Zero hunger chemistry.chemical_classification 0303 health sciences Multidisciplinary Indoleacetic Acids biology Hydrolysis fungi Chromosome Mapping food and beverages Biological Transport Starch Plants Genetically Modified biology.organism_classification chemistry Fermentation Mutation Carbohydrate Metabolism Medicine Plant hormone Polar auxin transport Research Article 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 8, Iss 1, p e55616 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0055616 |
| Popis: | With their unique metabolism and the potential to produce large amounts of biomass, plants are an excellent bio-energy feedstock for a variety of industrial purposes. Here we developed a high-throughput strategy, using the model plant Arabidopsis thaliana, to identify mutants with improved sugar release from plant biomass. Molecular analysis indicates a variety of processes including starch degradation, cell wall composition and polar transport of the plant hormone auxin can contribute to this improved saccharification. To demonstrate translatability, polar auxin transport in maize was either genetically or chemical inhibited and this also resulted in increased sugar release from plant tissues. Our forward genetic approach using Arabidopsis not only uncovers new functions that contribute to cell wall integrity but also demonstrates that information gleaned from this genetic model can be directly translated to monocotyledonous crops such as maize to improve sugar extractability from biomass. |
| Databáze: | OpenAIRE |
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