Cross-reaction of BK virus large T antigen with monoclonal antibodies directed against SV40 large T antigen
| Autor: | Robert S. Mann, Robert B. Carroll |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.drug_class
viruses Fluorescent Antibody Technique Simian virus 40 H antigen Biology Cross Reactions Immunofluorescence Monoclonal antibody Epitope Immunoenzyme Techniques Antigen Antibody Specificity Virology medicine Amino Acid Sequence Antigens Viral Tumor medicine.diagnostic_test T-cell receptor Antibodies Monoclonal Molecular biology Molecular Weight Immunoassay Macrophage-1 antigen BK Virus Polyomavirus |
| Zdroj: | Virology. 138(2) |
| ISSN: | 0042-6822 |
| Popis: | Seventy-five percent of the amino acid sequence of simian virus 40 (SV40) large T antigen is identical to that of the large T antigen of the human papovavirus, BK virus (BKV). Cross-reactivity between BKV T antigen and monoclonal antibodies directed against SV40 T antigen was studied by immunofluorescence, an enzyme immunoassay, immunoprecipitation of radiolabeled extracts, and Western blotting. BKV T antigen was found to be recognized by two monoclonal antibodies, PAb 416 and 430, which react with two distinct sites toward the amino terminus of SV40 large T antigen. These two sites may correspond to two hydrophilic regions of shared amino acid sequence which exist toward the amino termini of the T antigens. |
| Databáze: | OpenAIRE |
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