Beta-defensins and analogs in Helicobacter pylori infections: mRNA expression levels, DNA methylation, and antibacterial activity

Autor: Pero R., Angrisano T., Brancaccio M., Falanga A., Lombardi L., Natale F., Laneri S., Lombardo B., Galdiero S., Scudiero O., GALDIERO, STEFANIA
Přispěvatelé: Pero, R., Angrisano, T., Brancaccio, M., Falanga, A., Lombardi, L., Natale, F., Laneri, S., Lombardo, B., Galdiero, S., Scudiero, O., Galdiero, Stefania
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
beta-Defensins
Chronic gastritis
Pathology and Laboratory Medicine
Biochemistry
0302 clinical medicine
Helicobacter
Gene expression
Medicine and Health Sciences
Gastrointestinal Infections
Immune Response
Antiinfective agent
Multidisciplinary
DNA methylation
Microbial Sensitivity Test
Antimicrobials
Database and informatics methods
Sequence analysis
Drugs
Middle Aged
beta-Defensin
Chromatin
3. Good health
Bacterial Pathogens
Nucleic acids
medicine.anatomical_structure
Real-time polymerase chain reaction
Medical Microbiology
030220 oncology & carcinogenesis
Gastritis
Medicine
Epigenetics
Female
Pathogens
DNA modification
Chromatin modification
Human
Research Article
Chromosome biology
Adult
Cell biology
Bioinformatics
Science
Immunology
Gastroenterology and Hepatology
Microbial Sensitivity Tests
Biology
Real-Time Polymerase Chain Reaction
Microbiology
Helicobacter Infections
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Microbial Control
Gastric mucosa
medicine
Genetics
Humans
Microbial Pathogens
DNA sequence analysis
Inflammation
Pharmacology
Biology and life sciences
Bacteria
Dose-Response Relationship
Drug
Helicobacter pylori
Gastriti
Organisms
DNA
biology.organism_classification
medicine.disease
bacterial infections and mycoses
Research and analysis methods
030104 developmental biology
Beta defensin
Gastric Mucosa
Transcriptome
Helicobacter Infection
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 9, p e0222295 (2019)
ISSN: 1932-6203
Popis: Antimicrobial peptides can protect the gastric mucosa from bacteria, but Helicobacter pylori (H. pylori) can equally colonize the gastric apparatus. To understand beta-defensin function in H. pylori-associated chronic gastritis, we investigated susceptibility, human beta-defensin mRNA expression, and DNA methylation changes to promoters in the gastric mucosa with or without H. pylori infection. We studied the expression of HBD2 (gene name DEFB4A), HBD3 (DEFB103A), and HBD4 (DEFB104) using real-time PCR in 15 control and 10 H. pylori infection patient gastric specimens. This study demonstrates that H. pylori infection is related to gastric enhancement of inducible HBD2, but inducible HBD3 and HBD4 expression levels remained unchanged. HBD2 gene methylation levels were overall higher in H. pylori-negative samples than in H. pylori-positive samples. We also assessed antimicrobial susceptibility using growth on blood agar. The H. pylori strain Tox+ was susceptible to all defensins tested and their analogs (3N, 3NI). These results show that HBD2 is involved in gastritis development driven by H. pylori, which facilitates the creation of an epigenetic field during H. pylori-associated gastric tumorigenesis.
Databáze: OpenAIRE
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