Sex Hormones Coordinate Neutrophil Immunity in the Vagina by Controlling Chemokine Gradients
| Autor: | María Ángeles Muñoz-Fernández, Federico Perez-Millan, Teresa Navarro-González, Enrique Mercader, Laura Moreno-Ochoa, Miguel Relloso, Sandra Lasarte, Linnea A. Weiss, Paloma Sánchez-Mateos, Marjorie Pion, Elena Ceballos-García, Mauriel A. Guia-Gonzalez, Rafael Samaniego, Laura Salinas-Muñoz, Andrés Hidalgo |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Neutrophils Biology Transepithelial Migration Receptors Interleukin-8B 03 medical and health sciences Chemokine receptor Mice Cell Movement Internal medicine Candida albicans medicine Immunology and Allergy Animals Humans CCL15 Cells Cultured Progesterone Mice Knockout Candidiasis Estrogens respiratory system CCL20 CXCL2 030104 developmental biology Infectious Diseases Endocrinology medicine.anatomical_structure Gene Expression Regulation Vagina CXCL9 Female Chemokines CCL21 |
| Zdroj: | The Journal of infectious diseases. 213(3) |
| ISSN: | 1537-6613 |
| Popis: | Estradiol-based contraceptives and hormonal replacement therapy predispose women to Candida albicans infections. Moreover, during the ovulatory phase (high estradiol), neutrophil numbers decrease in the vaginal lumen and increase during the luteal phase (high progesterone). Vaginal secretions contain chemokines that drive neutrophil migration into the lumen. However, their expression during the ovarian cycle or in response to hormonal treatments are controversial and their role in vaginal defense remains unknown.To investigate the transepithelial migration of neutrophils, we used adoptive transfer of Cxcr2(-/-) neutrophils and chemokine immunofluorescence quantitative analysis in response to C. albicans vaginal infection in the presence of hormones.Our data show that the Cxcl1/Cxcr2 axis drives neutrophil transepithelial migration into the vagina. Progesterone promotes the Cxcl1 gradient to favor neutrophil migration. Estradiol disrupts the Cxcl1 gradient and favors neutrophil arrest in the vaginal stroma; as a result, the vagina becomes more vulnerable to pathogens. |
| Databáze: | OpenAIRE |
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