Melanin and humic acid-like polymer complex from olive mill waste waters. Part I. Isolation and characterization
| Autor: | Georgios Sotiroudis, Vassiliki Papadimitriou, Maissa Khemakhem, Nabiha Bouzouita, Cécile Arbez-Gindre, Theodore G. Sotiroudis, Panagiotis Zoumpoulakis |
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| Rok vydání: | 2016 |
| Předmět: |
Nitroxide mediated radical polymerization
Antioxidant medicine.medical_treatment Radical Industrial Waste Wastewater 010501 environmental sciences 01 natural sciences Antioxidants Analytical Chemistry chemistry.chemical_compound Biopolymers Cations Olea medicine Humic acid Humic Substances Plant Proteins 0105 earth and related environmental sciences Melanins chemistry.chemical_classification Chromatography ABTS 010401 analytical chemistry Cationic polymerization Polyphenols General Medicine Polymer 0104 chemical sciences chemistry Polyphenol Oxidation-Reduction Food Science |
| Zdroj: | Food Chemistry. 203:540-547 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2016.01.110 |
| Popis: | A water soluble humic acid and melanin-like polymer complex (OMWW-ASP) was isolated from olive mill waste waters (OMWW) by ammonium sulfate fractionation to be used as natural additive in food preparations. The dark polymer complex was further characterized by a variety of biochemical, physicochemical and spectroscopic techniques. OMWW-ASP is composed mainly of proteins associated with polyphenols and carbohydrates and the distribution of its relative molecular size was determined between about 5 and 190 kDa. SDS-PAGE shows the presence of a well separated protein band of 21.3 kDa and a low molecular weight peptide. The OMWW-ASP complex exhibits a monotonically increasing UV-Vis absorption spectrum and it contains stable radicals. Antioxidant activity measurements reveal the ability of the OMWW protein fraction to scavenge both the cationic 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)) radical, as well as the stable nitroxide free radical 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL). |
| Databáze: | OpenAIRE |
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