The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis

Autor: Candelario Vázquez-Cruz, Antonio Celestino-Montes, Hortensia Reyes-Cervantes, Estela Anastacio-Marcelino, Erasmo Negrete-Abascal, Dolores Bautista-España, Plinio Guzmán, Patricia Sánchez-Alonso, Guillermo Horta-Valerdi, Milorad Kojic
Rok vydání: 2014
Předmět:
Spores
Basidiomycetes
Telomerase
Ustilago
lcsh:Medicine
Yeast and Fungal Models
Biochemistry
Teliospore formation
Gene Expression Regulation
Fungal
Nucleic Acids
Molecular Cell Biology
lcsh:Science
Genetics
0303 health sciences
Multidisciplinary
biology
Chromosome Biology
030302 biochemistry & molecular biology
Genomics
Cell biology
Telomeres
Dimorphic fungus
Research Article
DNA Replication
Chromosome Structure and Function
Protein subunit
Saccharomyces cerevisiae
Mycology
Research and Analysis Methods
Microbiology
Chromosomes
Ustilago Maydis
03 medical and health sciences
Model Organisms
Telomerase reverse transcriptase
Amino Acid Sequence
030304 developmental biology
Biology and life sciences
lcsh:R
Organisms
Fungi
DNA structure
DNA
Cell Biology
biology.organism_classification
Diploidy
Telomere
lcsh:Q
Zdroj: PLoS ONE, Vol 9, Iss 10, p e109981 (2014)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0109981
Popis: In this study, we investigated the reverse transcriptase subunit of telomerase in the dimorphic fungus Ustilago maydis. This protein (Trt1) contains 1371 amino acids and all of the characteristic TERT motifs. Mutants created by disrupting trt1 had senescent traits, such as delayed growth, low replicative potential, and reduced survival, that were reminiscent of the traits observed in est2 budding yeast mutants. Telomerase activity was observed in wild-type fungus sporidia but not those of the disruption mutant. The introduction of a self-replicating plasmid expressing Trt1 into the mutant strain restored growth proficiency and replicative potential. Analyses of trt1 crosses in planta suggested that Trt1 is necessary for teliospore formation in homozygous disrupted diploids and that telomerase is haploinsufficient in heterozygous diploids. Additionally, terminal restriction fragment analysis in the progeny hinted at alternative survival mechanisms similar to those of budding yeast.
Databáze: OpenAIRE
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