Recruitment of endogenous progenitor cells by erythropoietin loaded particles for in situ cartilage regeneration
| Autor: | Baohong Yuan, Kayti Robinson, Joseph Borrelli, Liping Tang, Yihui Huang, Shuxin Li, Shuai Yu, Amirhossein Hakamivala |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0206 medical engineering
Biomedical Engineering 02 engineering and technology Article Biomaterials hemic and lymphatic diseases medicine lcsh:TA401-492 Progenitor cell Erythropoietin lcsh:QH301-705.5 Hyaluronic acid microscaffolds biology Regeneration (biology) Cartilage CD44 021001 nanoscience & nanotechnology Chondrogenesis Cartilage injury 020601 biomedical engineering Cell biology Transplantation medicine.anatomical_structure lcsh:Biology (General) biology.protein lcsh:Materials of engineering and construction. Mechanics of materials Stem cell Progenitor cells recruitment 0210 nano-technology Biotechnology medicine.drug |
| Zdroj: | Bioactive Materials, Vol 5, Iss 1, Pp 142-152 (2020) Bioactive Materials |
| Popis: | Cartilage injury affects millions of people throughout the world, and at this time there is no cure. While transplantation of stem cells has shown some success in the treatment of injured cartilage, such treatment is limited by limited cell sources and safety concerns. To overcome these drawbacks, a microscaffolds system was developed capable of targeting, reducing the inflammatory response and recruiting endogenous progenitor cells to cartilage-defect. Erythropoietin (EPO)-loaded-hyaluronic acid (HA) microscaffolds (HA + EPO) were fabricated and characterized. HA-microscaffolds showed good cell-compatibility and could target chondrocytes via CD44 receptors. HA + EPO was designed to slowly release EPO while recruiting progenitor cells. Finally, the ability of HA + EPO to repair cartilage-defects was assessed using a rabbit model of full-thickness cartilage-defect. Our results showed that the intra-articular administration of EPO, HA, and EPO + HA reduced the number of inflammatory cells inside the synovial-fluid, while EPO + HA had the greatest anti-inflammatory effects. Furthermore, among all groups, EPO + HA achieved the greatest progenitor cell recruitment and subsequent chondrogenesis. The results of this work support that, by targeting and localizing the release of growth-factors, HA + EPO can reduce inflammatory responses and promote progenitor cells responses. This new platform represents an alternative treatment to stem-cell transplantation for the treatment of cartilage injury. Graphical abstract Image 1 Highlights • Hyaluronic acid (HA) microscaffolds are designed to target injured and inflamed cartilage. • EPO loaded HA microscaffolds reduce inflammatory responses in Synovial fluid. • Released EPO promotes the recruitment of endogenous progenitor cells to the site of injured cartilage. • Released EPO increases chondrogenic differentiation of progenitor cells and leads to cartilage regeneration. |
| Databáze: | OpenAIRE |
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