Proliferation rate and penicillin binding of enterococcal isolates from patients with immunosupression
| Autor: | Ewa Bryl, Agnieszka Daca, Tomasz Jarzembowski, Jacek M. Witkowski |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Bacteriuria medicine.drug_class Antibiotics Population Microbial Sensitivity Tests Penicillins Biology 01 natural sciences Applied Microbiology and Biotechnology Asymptomatic Bacterial Adhesion Microbiology Immunocompromised Host 03 medical and health sciences Minimum inhibitory concentration 010608 biotechnology medicine Humans education Feces Cell Proliferation 0303 health sciences education.field_of_study 030306 microbiology medicine.disease Kidney Transplantation Transplant Recipients Anti-Bacterial Agents Penicillin Penicillin binding medicine.symptom Enterococcus medicine.drug |
| Zdroj: | Letters in Applied Microbiology. |
| ISSN: | 1472-765X 0266-8254 |
| DOI: | 10.1111/lam.13161 |
| Popis: | Low proliferation rate of bacterial populations was recently assumed to be a reason for higher resistance to antibiotics and appearance of many chronic infections. Slowly growing populations, called 'small colony variants' (SCVs) have been described in many bacterial species to make from as low as 0·02% up to 46% of population. Thirty enterococcal strains from urine and faeces of renal transplant recipients with asymptomatic, insignificant bacteriuria were studied. Growth characteristics were estimated by microculture and OD reading after 1, 3 and 5 h of culture. At the same time, penicillin binding and changes of aggregation of the cells were analysed by flow cytometry. The results of our study showed high diversity of the proliferation rates among studied isolates. Based on proliferation rates and aggregation, six of studied strains (20%) could be considered as SCVs-like. Significantly lower binding of penicillin was also observed for these SCV-like strains. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides relevant information about prevalence of enterococcal strains with low proliferation rate (likely small colony variant (SCV)) among kidney transplant recipients. Percentage of such strains in this cohort was relatively high (20%). Additionally, penicillin binding of these strains measured even at the beginning of proliferation (after 1 and 3 h of incubation), was significantly lower than among other strains. Finally, all of them were determined as penicillin resistant, with minimal inhibitory concentration value above 256 μg ml-1 . As the risk of systemic infections caused by such strains is probably higher than in case of other strains, screening for the SCVs in this group of patients should be recommended. |
| Databáze: | OpenAIRE |
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