Comparison of the immunomodulatory properties of three probiotic strains of Lactobacilli using complex culture systems: prediction for in vivo efficacy

Autor: Maria Rescigno, Erika Mileti, Iliyan D. Iliev, Gianluca Matteoli
Jazyk: angličtina
Rok vydání: 2009
Předmět:
medicine.drug_class
Antibiotics
Drug Evaluation
Preclinical
Immunology/Immunomodulation
lcsh:Medicine
Microbial Sensitivity Tests
Inflammatory bowel disease
law.invention
Microbiology
Mice
03 medical and health sciences
Probiotic
0302 clinical medicine
Species Specificity
Salmonella
law
In vivo
Immunology/Immunity to Infections
Lactobacillus
medicine
Animals
Humans
Colitis
lcsh:Science
030304 developmental biology
Inflammation
0303 health sciences
Multidisciplinary
biology
Probiotics
lcsh:R
Experimental colitis
food and beverages
Dendritic Cells
Th1 Cells
Inflammatory Bowel Diseases
biology.organism_classification
medicine.disease
digestive system diseases
3. Good health
Immunology/Immune Response
Immunology
Cytokines
030211 gastroenterology & hepatology
lcsh:Q
Lactobacillus plantarum
Research Article
Zdroj: PLoS ONE, Vol 4, Iss 9, p e7056 (2009)
PLoS ONE
ISSN: 1932-6203
Popis: BACKGROUND: While the use of probiotics to treat or prevent inflammatory bowel disease (IBD) has been proposed, to this point the clinical benefits have been limited. In this report we analyzed the immunological activity of three strains of Lactobacillus to predict their in vivo efficacy in protecting against experimental colitis. METHODOLOGY/PRINCIPAL FINDINGS: We compared the immunological properties of Lactobacillus plantarum NCIMB8826, L. rhamnosus GG (LGG), L. paracasei B21060 and pathogenic Salmonella typhimurium (SL1344). We studied the stimulatory effects of these different strains upon dendritic cells (DCs) either directly by co-culture or indirectly via conditioning of an epithelial intermediary. Furthermore, we characterized the effects of these strains in vivo using a Dextran sulphate sodium (DSS) model of colitis. We found that the three strains exhibited different abilities to induce inflammatory cytokine production by DCs with L. plantarum being the most effective followed by LGG and L. paracasei. L. paracasei minimally induced the release of cytokines, while it also inhibited the potential of DCs to both produce inflammatory cytokines (IL-12 and TNF-alpha) and to drive Th1 T cells in response to Salmonella. This effect on DCs was found under both direct and indirect stimulatory conditions - i.e. mediated by epithelial cells - and was dependent upon an as yet unidentified soluble mediator. When tested in vivo, L. plantarum and LGG exacerbated the development of DSS-induced colitis and caused the death of treated mice, while, conversely L. paracasei was protective. CONCLUSIONS: We describe a new property of probiotics to either directly or indirectly inhibit DC activation by inflammatory bacteria. Moreover, some immunostimulatory probiotics not only failed to protect against colitis, they actually amplified the disease progression. In conclusion, caution must be exercised when choosing a probiotic strain to treat IBD.
Databáze: OpenAIRE
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