Effects of vitamin A deficiency in the postnatal mouse heart: role of hepatic retinoid stores
| Autor: | Christine W. Duarte, Cécile Rochette-Egly, Amanda J. Favreau-Lessard, Mary Ann Asson-Batres, Sergey Ryzhov, Douglas B. Sawyer, Truc-Linh Tran, Oleg Tikhomirov, Craig R. Lessard, Clare Bates Congdon, Samuel McFarland, Cristi L. Galindo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Vitamin medicine.medical_specialty Microarray Receptors Retinoic Acid Physiology medicine.drug_class Myocardial Infarction Retinoic acid Mice Retinoids 03 medical and health sciences chemistry.chemical_compound Integrative Cardiovascular Physiology and Pathophysiology Physiology (medical) Internal medicine Animals Medicine Retinoid Myocardial infarction Progenitor cell Mice Knockout Vitamin A Deficiency business.industry Myocardium Retinol Heart medicine.disease Vitamin A deficiency 030104 developmental biology Endocrinology Liver chemistry Echocardiography Cardiology and Cardiovascular Medicine business Acyltransferases |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 310:H1773-H1789 |
| ISSN: | 1522-1539 0363-6135 |
| Popis: | To determine whether hepatic depletion of vitamin A (VA) stores has an effect on the postnatal heart, studies were carried out with mice lacking liver retinyl ester stores fed either a VA-sufficient (LRVAS) or VA-deficient (LRVAD) diet (to deplete circulating retinol and extrahepatic stores of retinyl esters). There were no observable differences in the weights or gross morphology of hearts from LRVAS or LRVAD mice relative to sex-matched, age-matched, and genetically matched wild-type (WT) controls fed the VAS diet (WTVAS), but changes in the transcription of functionally relevant genes were consistent with a state of VAD in LRVAS and LRVAD ventricles. In silico analysis revealed that 58/67 differentially expressed transcripts identified in a microarray screen are products of genes that have DNA retinoic acid response elements. Flow cytometric analysis revealed a significant and cell-specific increase in the number of proliferating Sca-1 cardiac progenitor cells in LRVAS animals relative to WTVAS controls. Before myocardial infarction, LRVAS and WTVAS mice had similar cardiac systolic function and structure, as measured by echocardiography, but, unexpectedly, repeat echocardiography demonstrated that LRVAS mice had less adverse remodeling by 1 wk after myocardial infarction. Overall, the results demonstrate that the adult heart is responsive to retinoids, and, most notably, reducing hepatic VA stores (while maintaining circulating levels of VA) impacts ventricular gene expression profiles, progenitor cell numbers, and response to injury. |
| Databáze: | OpenAIRE |
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