Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria
| Autor: | Elizabeth S. McKenney, Joanna B. Goldberg, Herbert P. Schweizer, F. Heath Damron |
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| Rok vydání: | 2012 |
| Předmět: |
Genetics
Genetics Microbial Gram-negative bacteria Ecology biology Genetic Vectors Mutagenesis (molecular biology technique) Gene Expression biology.organism_classification Applied Microbiology and Biotechnology Host Specificity Mutagenesis Insertional Gene cassette Gene expression Gram-Negative Bacteria DNA Transposable Elements Methods Heterologous expression Expression cassette Promoter Regions Genetic Gene Bacteria Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 79(2) |
| ISSN: | 1098-5336 |
| Popis: | We describe a mini-Tn 7 -based broad-host-range expression cassette for arabinose-inducible gene expression from the P BAD promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host. |
| Databáze: | OpenAIRE |
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