Anti-apoptotic effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) polymerase, against multiple organ damage induced by gamma irradiation in rats
| Autor: | Aiman S. El-Khatib, Rania M. El-Hazek, Marwa M. El-Sheikh, Mona A. El-Ghazaly |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Thiobarbituric acid Poly ADP ribose polymerase Apoptosis Poly(ADP-ribose) Polymerase Inhibitors Pharmacology Kidney medicine.disease_cause Poly (ADP-Ribose) Polymerase Inhibitor 03 medical and health sciences chemistry.chemical_compound Chlorocebus aethiops TBARS medicine Animals Radiology Nuclear Medicine and imaging Rats Wistar Vero Cells Dose-Response Relationship Drug Radiological and Ultrasound Technology biology Rats Oxidative Stress 030104 developmental biology Liver chemistry Gamma Rays 3-Aminobenzamide Myeloperoxidase Benzamides biology.protein Oxidative stress |
| Zdroj: | International Journal of Radiation Biology. 94:45-53 |
| ISSN: | 1362-3095 0955-3002 |
| Popis: | PURPOSE This study aimed to investigate the effect of 3-aminobenzamide (3-AB) in doses of 5, 10 and 15 mg/kg on the inhibition of Poly (ADP-ribose) polymerase (PARP) when combined with ionizing radiation (IR). MATERIAL AND METHODS Rats were treated intraperitonealy, one hour prior to irradiation at a dose level of 6 Gray (Gy) and were sacrificed 24 hours after irradiation. Control groups were run concurrently. RESULTS IR led to an increase of thiobarbituric acid reactive substance (TBARS), nitrite as well as a decrease in total antioxidant capacity associated increase in myeloperoxidase (MPO) with the expression of cyclooxygenase-2 (COX-2). Moreover, IR caused an increase in serum lactate dehydrogenase (LDH) activity and cytosolyic Ca+2 associated with an expression of Caspase-3 as well as a decline in complex-I activity and adenosine triphosphate (ATP) level. Pretreatment with 5 and 10 mg/kg of 3AB guarded against the changes in all the measured parameters, conversely the dose of 15 mg/kg showed no effect on the damage induced by irradiation in the selected tissues. Moreover, 3AB has a dose-dependent effect on viability of Vero cells. CONCLUSION The selected low doses of 3AB rather than the higher dose (15 mg/kg) protected against radiation-induced multiple organ damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|