Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs
| Autor: | Stella Diafa, Christian J. Leumann, Marcel Hollenstein, Damien Evéquoz |
|---|---|
| Přispěvatelé: | University of Bern, Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by Institut Pasteur start-up funds to MH and the Swiss National Science Foundation (grant n°: 200020-146646). |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
biology
Solid-phase synthesis 010405 organic chemistry Oligonucleotide DNA polymerase [CHIM.ORGA]Chemical Sciences/Organic chemistry Aptamer XNA Chemical modification 010402 general chemistry 01 natural sciences Combinatorial chemistry 0104 chemical sciences chemistry.chemical_compound DNA polymerases chemistry Nucleoside triphosphate Nucleic acid biology.protein [CHIM]Chemical Sciences Primer extension reactions Bicyclo-DNA Nucleoside |
| Zdroj: | Non-Natural Nucleic Acids Non-Natural Nucleic Acids, 1973, Humana, pp.1-13, 2019, Methods in Molecular Biology, ⟨10.1007/978-1-4939-9216-4_1⟩ Methods in Molecular Biology ISBN: 9781493992157 |
| DOI: | 10.1007/978-1-4939-9216-4_1⟩ |
| Popis: | International audience; Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP. |
| Databáze: | OpenAIRE |
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