Dengue Virus Infection of Primary Human Smooth Muscle Cells
| Autor: | Laya Hun, Rodrigo Mora-Rodriguez, Gilbert D. Loría, Jorge L. Arias-Arias, Eugenia Corrales-Aguilar, Francisco Vega-Aguilar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Permissiveness Vascular smooth muscle viruses 030231 tropical medicine Myocytes Smooth Muscle Primary Cell Culture Viral Plaque Assay Vascular problems Dengue virus Biology Infections medicine.disease_cause Kidney Serogroup Virus Replication Umbilical vein Umbilical Arteries Dengue fever Cell Line Dengue Pathogenesis 03 medical and health sciences 0302 clinical medicine Virology medicine Human Umbilical Vein Endothelial Cells Myocyte Animals Humans Epithelial Cells Articles Dengue Virus Viral Load medicine.disease Macaca mulatta 030104 developmental biology Infectious Diseases Smooth muscle cells Viral replication Parasitology |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene, vol.99(6), pp. 1451-1457 Kérwá Universidad de Costa Rica instacron:UCR |
| Popis: | Dengue virus (DENV) infection of humans is presently the most important arthropod-borne viral global threat, for which no suitable or reliable animal model exists. Reports addressing the effect of DENV on vascular components other than endothelial cells are lacking. Dengue virus infection of vascular smooth muscle cells, which play a physiological compensatory response to hypotension in arteries and arterioles, has not been characterized, thus precluding our understanding of the role of these vascular components in dengue pathogenesis. Therefore, we studied the permissiveness of primary human umbilical artery smooth muscle cells (HUASMC) to DENV 1-4 infection and compared with the infection in the previously reported primary human umbilical vein endothelial cells (HUVEC) and the classically used, non-transformed, and highly permissive Lilly Laboratories Cell-Monkey Kidney 2 cells. Our results show that HUASMC are susceptible and productive to infection with the four DENV serotypes, although to a lesser extent when compared with the other cell lines. This is the first report of DENV permissiveness in human smooth muscle cells, which might represent an unexplored pathophysiological contributor to the vascular collapse observed in severe human dengue infection. Universidad de Costa Rica/[803-A5-025]/UCR/Costa Rica UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|