High hydrostatic pressure treatment impairs AcrAB-TolC pump resulting in differential loss of deoxycholate tolerance in Escherichia coli
| Autor: | Rikizo Aono, Taketo Kawarai, Makari Yamasaki, Ayako Irie, Yoshinori Inagi, Akari Ida, Mami Kishima, Hirokazu Ogihara, Soichi Furukawa |
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| Rok vydání: | 2005 |
| Předmět: |
Cell Survival
Tetracycline Hydrostatic pressure Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Bacterial Proteins Drug Resistance Bacterial Hydrostatic Pressure medicine Escherichia coli Escherichia coli K12 biology Chemiosmosis Transporter biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae chemistry Biophysics bacteria Carrier Proteins Ethidium bromide Bacteria Deoxycholic Acid Biotechnology medicine.drug |
| Zdroj: | Journal of Bioscience and Bioengineering. 100:613-616 |
| ISSN: | 1389-1723 |
| Popis: | We reported previously that high hydrostatic pressure-injured stationary phase cells of Escherichia coli K-12 lost their intrinsic deoxycholate tolerance. The AcrAB-TolC multi-drug resistance pump driven by proton motive force has been argued to be responsible for the tolerance to deoxycholate. In this report, we tested the sensitivity of the AcrAB-TolC (three components) pump to high hydrostatic pressure treatment (HPT). E. coli K-12 treated with HPT became sensitive to AcrAB-TolC-specific drugs such as ethidium bromide, but not to tetracycline which is pumped out by a one-component transporter, Tet. Only E. coli K-12 overproducing both AcrAB and TolC exhibited restored tolerance to deoxycholate after HPT but not E. coli overproducing either TolC or AcrAB. These observations strongly suggest that three-component pumps such as AcrAB-TolC are more susceptible to HPT than one-component pumps such as Tet, resulting in the differential loss of deoxycholate tolerance in high hydrostatic pressure-injured E. coli cells. |
| Databáze: | OpenAIRE |
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