Myogenic differentiation 1 and transcription factor 12 activate the gene expression of mouse taste receptor type 1 member 1
| Autor: | Shoichiro Kokabu, Shinji Kataoka, Yui Obikane, Mitsushiro Nakatomi, Kae Matsuyama, Ryuji Hosokawa, Takashi Toyono, Yuji Seta |
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| Rok vydání: | 2021 |
| Předmět: |
animal structures
Medicine (miscellaneous) Gene Expression Promoter Muscle Development General Biochemistry Genetics and Molecular Biology Cell biology chemistry.chemical_compound Mice chemistry Taste embryonic structures Gene expression Transcriptional regulation Animals Binding site General Dentistry Chromatin immunoprecipitation Gene Transcription factor DNA MyoD Protein Transcription Factors |
| Zdroj: | Journal of oral biosciences. 63(4) |
| ISSN: | 1880-3865 |
| Popis: | Objectives Myogenic differentiation 1 (Myod1) is involved in the expression of taste receptor type 1 member 1 (Tas1r1) during myogenic differentiation. Further, the target genes of Myod1 participate in transcriptional control, muscle development, and synaptic function. We examined, for the first time, the function of Myod1 in the transcriptional regulation of Tas1r1. Methods ENCODE chromatin immunoprecipitation and sequencing (ChIP-seq) data of myogenically differentiated C2C12 cells were analyzed to identify the Myod1 and transcription factor 12 (Tcf12) binding sites in the Tas1r1 promoter region. Luciferase reporter assays, DNA affinity precipitation assays, and co-immunoprecipitation assays were also performed to identify the functions of Myod1, Tcf12, and Kruppel-like factor 5 (Klf5). Results Based on ENCODE ChIP-seq, Myod1 bound to the Tas1r1 promoter region containing E-boxes 1–3. Luciferase reporter assays revealed that site-directed E-box1 mutations significantly reduced promoter activation induced by Myod1 overexpression. According to the DNA affinity precipitation assay and co-immunoprecipitation assay, Myod1 formed a heterodimer with Tcf12 and bound to E-box1. Further, Klf5 bound to the GT box near E-box1, activating Tas1r1 expression. Conclusions During myogenic differentiation, the Myod1/Tcf12 heterodimer, in collaboration with Klf5, binds to E-box1 and activates Tas1r1 expression. |
| Databáze: | OpenAIRE |
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