Regulatory functions of the three nodD genes of Rhizobium leguminosarum biovar phaseoli
| Autor: | E. O. Davis, A. W. B. Johnston |
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| Rok vydání: | 1990 |
| Předmět: |
Exudate
Rhizobiaceae Transcription Genetic Operon animal diseases Biovar Mutant Restriction Mapping medicine.disease_cause N-Acetylglucosaminyltransferases Microbiology Rhizobium leguminosarum Bacterial Proteins Transcription (biology) Nitrogen Fixation medicine Molecular Biology Gene Plants Medicinal biology food and beverages Fabaceae Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification beta-Galactosidase Lac Operon Genes Bacterial Mutation bacteria medicine.symptom Bacterial Outer Membrane Proteins Plasmids Rhizobium |
| Zdroj: | Molecular microbiology. 4(6) |
| ISSN: | 0950-382X |
| Popis: | Summary The three nodD genes of a strain of Rhizobium leguminosarum biovar phaseoli were cloned to study their effects on transcription of themselves and of the nodC genes of biovars phaseoli and viciae. Efficient transcription of nodD1 required nodD1 and was enhanced by exposure of the cells to bean exudate consistent with the presence of a nod-box preceding the nolE-nodD1 operon. Transcription of nodD2 and nodDZ was constitutive. nodC of R. leguminosarum biovar phaseoli was activated by each of the nodD genes of that biovar in the absence of inducers but expression was enhanced in cells grown with bean exudate or the flavonoids genistein or naringenin. A mutant of nodD2, tacking 60bp at its 3’end, activated nodC in the presence of inducer, but was defective in regulating certain of the nodD genes. The nodC gene of R. leguminosarum biovar viciae responded differently to the various nodD genes of R. leguminosarum biovar phaseoli than did the nodC of the latter biovar. |
| Databáze: | OpenAIRE |
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