Indole-3-carbinol activates the cyclin-dependent kinase inhibitor p15INK4b gene
| Autor: | Youichirou Matsuzaki, Toshiaki Hitomi, Makoto Koyama, Mayumi Kawanaka, Toshiyuki Sakai |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Keratinocytes
Indoles Biophysics Down-Regulation Cell Cycle Proteins p15INK4b Biochemistry Indole-3-carbinol Structural Biology Cyclin-dependent kinase Genetics Anticarcinogenic Agents Humans Genes Tumor Suppressor RNA Messenger Promoter Regions Genetic Molecular Biology Cell Line Transformed Cyclin-Dependent Kinase Inhibitor p15 Cyclin-dependent kinase 1 biology Dose-Response Relationship Drug G1 arrest Cyclin-dependent kinase 4 Tumor Suppressor Proteins HaCaT Cyclin-dependent kinase 2 Cyclin-dependent kinase 3 G1 Phase Promoter Cell Biology Molecular biology Cyclin-Dependent Kinases Kinetics Gene Expression Regulation biology.protein Cyclin-dependent kinase 6 RB Restriction point Cyclin A2 |
| Zdroj: | FEBS Letters. (1-2):137-140 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2004.09.002 |
| Popis: | Indole-3-carbinol (I3C) is a naturally occurring compound found in vegetables such as broccoli and cauliflower, and has been shown to arrest human tumor cells in the G1 phase of the cell cycle. However, the molecular mechanism responsible for this effect has not been sufficiently elucidated. We report here that I3C activates the cyclin-dependent kinase (CDK) inhibitor p15INK4b gene through its promoter, accompanied by cell growth inhibition in HaCaT cells. Treatment with I3C almost did not affect the expressions of the other CDK inhibitors such as p19INK4d, p21WAF1 and p27Kip1. These results suggest that p15INK4b is an important molecular target of I3C among CDK inhibitors. |
| Databáze: | OpenAIRE |
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