Transcriptome profiling of visceral adipose tissue in a novel obese rat model, WNIN/Obits comparison with other animal models
| Autor: | Siva Sankara Vara Prasad Sakamuri, Uday Kumar Putcha, Vajreswari Ayyalasomayajula, Giridharan Nappan Veettil |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty obesity Microarray Adipose tissue lcsh:Medicine Type 2 diabetes WNIN/Ob obese rat Biology Intra-Abdominal Fat General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Downregulation and upregulation Internal medicine Lipid biosynthesis Gene expression medicine Animals Humans RNA Small Nucleolar visceral adipose tissue 030109 nutrition & dietetics Microarray - obesity - small nucleolar RNA - visceral adipose tissue - WNIN/Ob obese rat lcsh:R 0402 animal and dairy science small nucleolar RNA Histology 04 agricultural and veterinary sciences General Medicine medicine.disease 040201 dairy & animal science Obesity Immunity Innate Rats Disease Models Animal Endocrinology Gene Expression Regulation Liver Tissue Array Analysis Original Article Transcriptome Metabolic Networks and Pathways |
| Zdroj: | The Indian Journal of Medical Research Indian Journal of Medical Research, Vol 144, Iss 3, Pp 409-423 (2016) |
| ISSN: | 0971-5916 |
| Popis: | Background & objectives: Adipose tissue dysfunction in obesity is linked to the development of type 2 diabetes and cardiovascular diseases. We studied the differential gene expression in retroperitoneal adipose tissue of a novel obese rat model, WNIN/Ob, to understand the possible underlying transcriptional changes involved in the development of obesity and associatedcomorbidities in this model. Methods: Four month old, male WNIN/Ob lean and obese rats were taken, blood was collected and tissues were dissected. Body composition analysis and adipose tissue histology were performed. Global gene expression in retroperitoneal adipose tissue of lean and obese rats was studied by microarray using Affymetrix GeneChips. Results: One thousand and seventeen probe sets were downregulated and 963 probe sets were upregulated (more than two-fold) in adipose tissue of WNIN/Ob obese rats when compared to that of lean rats. Small nucleolar RNA (SnoRNA) made most of the underexpressed probe sets, whereas immune system-related genes werethe most overexpressed in the adipose tissues of obese rats. Genes coding for cytoskeletal proteinswere downregulated, whereas genes related to lipid biosynthesis were elevated in the adipose tissue of obese rats. Interpretation & conclusions: Majority of the altered genes and pathways in adipose tissue of WNIN/Ob obese rats were similar to the observations in other obese animal models and human obesity. Based on these observations, it is proposed that WNIN/Ob obese rat model may be a good model to study the mechanisms involved in the development of obesity and its comorbidities. Downregulation of SnoRNA appears to be a novel feature in this obese rat model. |
| Databáze: | OpenAIRE |
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