Mössbauer spectroscopy of haemoglobin derivatives
| Autor: | G Lang, C E Johnson, M R Winter, J P Williams |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Azides
Spin states Iron Biochemistry Genetics and Molecular Biology (miscellaneous) Spectral line law.invention chemistry.chemical_compound Hemoglobins Magnetics Nuclear magnetic resonance law Mössbauer spectroscopy Nitriles Methods Animals Sulfhydryl Compounds Electron paramagnetic resonance Spin (physics) Cyanates Methemoglobin Magnetic moment Spectrum Analysis Imidazoles Quadrupole splitting Fluorine Cyanate Iron Isotopes Rats Cold Temperature Cobalt Isotopes chemistry Chromatography Gel Physical chemistry Condensed Matter::Strongly Correlated Electrons Oxidation-Reduction |
| Zdroj: | Biochimica et biophysica acta. 263(3) |
| ISSN: | 0006-3002 |
| Popis: | 1. 1. The Mossbauer spectra of the fluoride, cyanide, azide, imidazole, cyanate and methane thiol derivatives of iron(III) haemoglobin (methaemoglobin) have been measured over a range of temperature from 4 to 195 °K. 2. 2. In favourable cases, measurements at the higher temperatures allow us to distinguish decisively between the high- and low-spin forms of the compounds. 3. 3. Changes in isomer shift from compound to compound and from spin state to spin state are relatively small. Variations in quadrupole splitting are more appreciable and depend greatly on the nature of the axial ligand. 4. 4. Measurement of the Mossbauer spectra in a magnetic field at 4.2 °K provides a more direct method of spin state determination. 5. 5. It is shown that the Mossbauer spectra of methaemoglobin derivatives of intermediate magnetic moment may be approximated by the addition of appropriate amounts of the spectra of a purely high-spin and a purely low-spin derivative. 6. 6. From these magnetic field data we propose a set of empirical rules for the determination of spin composition in iron(III) haem compounds. |
| Databáze: | OpenAIRE |
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