Histopathological correlations of islet amyloidosis with apolipoprotein E polymorphisms in type 2 diabetic Chinese patients
| Autor: | Yi Sui, Heung Man Lee, Hai-Lu Zhao, Fernand Mac-Moune Lai, Juliana C.N. Chan, Peter C.Y. Tong, Lan He, Jing Guan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Apolipoprotein E
Male Pathology medicine.medical_specialty China Arteriosclerosis Endocrinology Diabetes and Metabolism Type 2 diabetes Islets of Langerhans Endocrinology Apolipoproteins E Asian People Polymorphism (computer science) Diabetes mellitus Internal Medicine medicine Humans Aged Aged 80 and over Polymorphism Genetic Hepatology business.industry Amyloidosis Case-control study Pancreatic Diseases Middle Aged medicine.disease Islet Amyloid Polypeptide Serum Amyloid P-Component Diabetes Mellitus Type 2 Case-Control Studies lipids (amino acids peptides and proteins) Female Alzheimer's disease business |
| Zdroj: | ResearcherID |
| ISSN: | 1536-4828 |
| Popis: | Islet amyloidosis and arteriosclerosis are histopathological hallmarks in type 2 diabetes. Apolipoprotein E (ApoE) is a common component of amyloidosis. ApoE [Latin Small Letter Open E]4 allele is associated with arteriosclerosis and cerebral amyloidosis in Alzheimer disease. We examined the correlations of ApoE polymorphisms with islet amyloidosis in type 2 diabetes.Genomic DNA samples were obtained from 117 autopsy cases with type 2 diabetes and 209 nondiabetic cases. ApoE genotypes and amylin gene mutations were determined by polymerase chain reaction-ligase detection reaction analysis. Islet amyloidosis and arteriosclerosis were evaluated by staining of thioflavin T, amylin, ApoE, and amyloid P component.In the diabetic group, 33.3% in group [Latin Small Letter Open E]2 ([Latin Small Letter Open E]2[Latin Small Letter Open E]2, [Latin Small Letter Open E]2[Latin Small Letter Open E]3), 23.6% in group [Latin Small Letter Open E]3 ([Latin Small Letter Open E]3[Latin Small Letter Open E]3), and 62.5% in group [Latin Small Letter Open E]4 ([Latin Small Letter Open E]4[Latin Small Letter Open E]4, [Latin Small Letter Open E]3[Latin Small Letter Open E]4) had islet amyloidosis. After adjustment for confounders, group [Latin Small Letter Open E]4 had an odds ratio of 7.0 (95% confidence interval, 1.3-38.0; P = 0.023) in having islet amyloidosis compared to group [Latin Small Letter Open E]3. Diabetic cases with islet amyloidosis had more severe arteriosclerosis (P = 0.0111), arteriolar hyalinosis (P = 0.0369), and interstitial fibrosis (P = 0.0188) than those without amyloidosis. Immunoreactivity of both ApoE and amyloid P component was detected in islet amyloid deposits and arteriosclerotic lesions.In type 2 diabetes, islet amyloidosis and arteriosclerosis share common pathophysiological features with ApoE [Latin Small Letter Open E]4 as a probable linking factor. |
| Databáze: | OpenAIRE |
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