Spectroscopic Evaluation of DNA–Borate Interactions
| Autor: | Turgay Tekinay, Omer Faruk Sarioglu, Ayse Ozdemir |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Circular dichroism
Unclassified drug Endocrinology Diabetes and Metabolism Clinical Biochemistry Biochemistry Colemanite chemistry.chemical_compound Chemical structure Ethidium Borates Spectroscopy Fourier Transform Infrared Ultraviolet spectroscopy Organic chemistry Calf thymus DNA Infrared spectroscopy Protein secondary structure Spectroscopy Sugar phosphate Competitive binding assay Circular Dichroism General Medicine Boric acid Absorption spectroscopy Deoxyribose sugar Thymus Gland engineering.material Interactions with DNA Inorganic Chemistry Ulexite Animals DNA helix DNA binding Boron Ligand binding assay Biochemistry (medical) DNA structure DNA Crystallography chemistry engineering Nucleic Acid Conformation Cattle Spectrophotometry Ultraviolet Controlled study |
| Zdroj: | Biological Trace Element Research |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/s12011-015-0369-5 |
| Popis: | We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV–vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding. © 2015, Springer Science+Business Media New York. |
| Databáze: | OpenAIRE |
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