Expression of prostaglandin H synthase isoforms in human myometrium at parturition
| Autor: | Leslie Myatt, S. D. Moore, S. E. Burk, D. M. Slater, L. M. Cornelison, Julia Brodt-Eppley |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
medicine.medical_specialty Uterus Prostaglandin Biology Polymerase Chain Reaction Exon chemistry.chemical_compound Pregnancy Internal medicine medicine Humans RNA Messenger chemistry.chemical_classification Labor Obstetric Myometrium Membrane Proteins Obstetrics and Gynecology Isoenzymes Endocrinology Enzyme medicine.anatomical_structure chemistry Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases In utero RNA splicing Cyclooxygenase 1 Female lipids (amino acids peptides and proteins) |
| Zdroj: | American Journal of Obstetrics and Gynecology. 180:103-109 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/s0002-9378(99)70157-2 |
| Popis: | The purpose of this study was to identify the isoforms and splicing patterns of prostaglandin H synthase present in pregnant human lower-segment myometrium and determine whether there is differential expression of the isoforms or splice variants with respect to gestational age or parturition.Lower-segment myometrium was collected at cesarean section at term (37 weeks) or preterm (37 weeks) from patients who were or were not in labor. Total messenger ribonucleic acid was isolated and reverse transcribed. Polymerase chain reaction for prostaglandin H synthase isoforms 1 and 2 and calponin were performed. Primers designed to characterize the splicing patterns of exon 9 of prostaglandin H synthase-1 were used.The predominant polymerase chain reaction product in all samples corresponds to prostaglandin H synthase-1 messenger ribonucleic acid spliced to include exon 9, but a less-abundant polymerase chain reaction product corresponding to prostaglandin H synthase-1 messenger ribonucleic acid spliced at the internal donor site of exon 9 was also detected. Prostaglandin H synthase-2 messenger ribonucleic acid was detected in human myometrium at a lower abundance than prostaglandin H synthase-1, and neither prostaglandin H synthase-1 or prostaglandin H synthase-2 messenger ribonucleic acid expression changed significantly with gestational age or labor.Both prostaglandin H synthase-1 and prostaglandin H synthase-2 isoforms are present in human myometrium. The prostaglandin H synthase-1 messenger ribonucleic acid that includes all of exon 9 encodes the predominant prostaglandin H synthase-1 isoform present in human myometrium. No significant alterations in the expression or splicing patterns for prostaglandin H synthase-1 were detected with respect to gestational age or the onset of labor; but prostaglandin H synthase-1 expression appeared higher at term in anticipation of labor. Although prostaglandin H synthase-2 is present in human myometrium, induction of prostaglandin H synthase-2 does not occur in lower-segment myometrium at parturition. |
| Databáze: | OpenAIRE |
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