miR-146a Controls Immune Response in the Melanoma Microenvironment
| Autor: | Heide Dierbach, Justin Mastroianni, Annette Schmitt-Graeff, Martina Falk, Natalie Stickel, Dietmar Pfeifer, Melanie Boerries, Kathrin Hanke, Wolfgang Melchinger, Sandra Duquesne, Dominik Schmidt, Frank Meiss, Hana Andrlová, Geoffroy Andrieux, Robert Zeiser |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_treatment Biology B7-H1 Antigen Article Interferon-gamma Mice 03 medical and health sciences 0302 clinical medicine Immune system Cell Movement Interferon Tumor Cells Cultured Tumor Microenvironment medicine Animals Humans Interferon gamma Melanoma Skin Mice Knockout Regulation of gene expression Cell migration Cell cycle Prognosis medicine.disease 3. Good health Gene Expression Regulation Neoplastic Mice Inbred C57BL MicroRNAs STAT1 Transcription Factor 030104 developmental biology Cytokine Oncology Case-Control Studies 030220 oncology & carcinogenesis Cancer research medicine.drug |
| Zdroj: | Cancer Research. 79:183-195 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | MicroRNAs (miR) are small noncoding RNAs that regulate gene expression, posttranscription, and manipulate immune responses in different types of cancers. In this study, we identify miR-146a as a negative regulator of immune activation, comparable to immune-checkpoint molecules. miR-146a levels were increased in melanoma microenvironmental tissue, and miR-146a−/− mice survived longer and developed less metastases in comparison with wild-type melanoma-bearing mice. T cells isolated from miR-146a−/− mice revealed higher expression levels of the miR-146a target gene Stat1 and the Stat1-regulated cytokine IFNγ. Neutralization of IFNγ in miR-146a−/− mice decreased survival and increased melanoma metastasis patterns to those of wild-type mice. In vitro, IFNγ reduced melanoma cell migration, cell-cycle activity, and basal metabolic rate. Conversely, IFNγ also increased PD-L1 levels on the melanoma cells, which may counterbalance some of the beneficial effects increasing immune escape in vivo. Combined treatment with a miR-146a antagomiR and anti–PD-1 resulted in improved survival over isotype control or anti–PD-1 treatment alone. In summary, these data show that miR-146a plays a central role within the STAT1/IFNγ axis in the melanoma microenvironment, affecting melanoma migration, proliferation, and mitochondrial fitness as well as PD-L1 levels. Additionally, combined inhibition of PD-1 and miR-146a could be a novel strategy to enhance antitumor immune response elicited by checkpoint therapy. Significance: These findings identify a microRNA–based mechanism by which melanoma cells escape the immune system, providing a new therapeutic strategy to improve the current management of patients with melanoma. |
| Databáze: | OpenAIRE |
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