Phagocytosis of fibronectin and the collagens type I, III and V by human gingival and periodontal ligament fibroblasts in vitro
| Autor: | T. van den Bos, Vincent Everts, Wouter Beertsen, M.T.M. van der Pauw |
|---|---|
| Přispěvatelé: | Other departments, Parodontologie (OUD, ACTA) |
| Rok vydání: | 2001 |
| Předmět: |
Adult
Adolescent Cytochalasin B Periodontal Ligament Phagocytosis Statistics as Topic Gingiva Collagen Type I Flow cytometry Extracellular matrix Dental Enamel Proteins stomatognathic system medicine Periodontal fiber Animals Humans Bovine serum albumin Cells Cultured Analysis of Variance biology medicine.diagnostic_test Chemistry General Engineering Serum Albumin Bovine DNA Fibroblasts Alkaline Phosphatase Flow Cytometry Molecular biology In vitro Fibronectins Fibronectin Actin Cytoskeleton Microscopy Electron Collagen Type III Immunology biology.protein Periodontics Alkaline phosphatase Cattle Collagen Type V |
| Zdroj: | Journal of periodontology, 72(10), 1340-1347. American Academy of Periodontology van der Pauw, M T M, van den Bos, T, Everts, V & Beertsen, W 2001, ' Phagocytosis of fibronectin and the collagens type I, III and V by human gingival and periodontal ligament fibroblasts in vitro ', Journal of Periodontology, vol. 72, pp. 1340-1347 . https://doi.org/10.1902/jop.2001.72.10.1340 Journal of Periodontology, 72, 1340-1347. John Wiley & Sons Inc. Journal of Periodontology, 72, 1340-1347. American Academy of Periodontology |
| ISSN: | 0022-3492 |
| Popis: | Electron microscopic studies have suggested that the volume density of collagen-containing vacuoles in fibroblasts is higher in the periodontal ligament (PDL) than in the gingiva. Whether this difference reflects intrinsic differences in phagocytic capacity among the cells in these tissues is not known. PDL and gingival fibroblasts were isolated from subjects and cultured under identical conditions in the presence of fluorescent beads coated with collagen type I, III, or V or fibronectin. Control beads were coated with bovine serum albumin or an enamel matrix protein mixture that does not constitute part of the extracellular matrix of PDL and gingiva. After various time intervals (1 to 24 hours), the percentage of cells that had internalized beads was assessed by flow cytometry. Since alkaline phosphatase activity has been suggested to play a role in collagen phagocytosis, the activity of this enzyme was determined for all cell populations. The results demonstrated the following order in the percentage of cells internalizing protein-coated beads: fibronectin > collagen type I > III > V. Internalization of collagen type I-coated beads exceeded that of beads coated with bovine serum albumin or enamel matrix proteins by 6 and 3 times, respectively. No differences were observed in collagen phagocytic activity between PDL and gingival fibroblasts, and no relationship could be demonstrated between collagen phagocytosis and alkaline phosphatase activity. We conclude that differences in collagen phagocytosis between PDL and gingiva, as observed in vivo, are not likely to be explained in terms of intrinsic phagocytic capacities of these cells |
| Databáze: | OpenAIRE |
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