Premature chromatid separation and altered proliferation of human leukocytes treated with vanadium (III) oxide

Autor: Mario Altamirano-Lozano, Rodrigo Anibal Mateos-Nava, Juan José Rodríguez-Mercado
Rok vydání: 2016
Předmět:
Adult
DNA Replication
Male
0301 basic medicine
Vanadium Compounds
Premature chromatid separation
Mitotic index
Cell Survival
Health
Toxicology and Mutagenesis
Centromere
Vanadium
chemistry.chemical_element
Sister chromatid exchange
Chromatids
Biology
Toxicology
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Leukocytes
Mitotic Index
Humans
Viability assay
Vanadium(III) oxide
Cells
Cultured
Cell Proliferation
Pharmacology
Genetics
Chemical Health and Safety
Mutagenicity Tests
Cell growth
Cell Cycle
Osmolar Concentration
Public Health
Environmental and Occupational Health
Oxides
General Medicine
Aneugens
Molecular biology
Carcinogens
Environmental
In vitro
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Sister Chromatid Exchange
Zdroj: Drug and Chemical Toxicology. 40:457-462
ISSN: 1525-6014
0148-0545
DOI: 10.1080/01480545.2016.1260582
Popis: Vanadium is a widely distributed metal in the Earth’s surface and is released into the environment by either natural or anthropogenic causes. Vanadium (III) oxide (V2O3) is present in the environment, and many organisms are exposed to this compound; however, its effects at the cellular and genetic levels are still unknown. Therefore, in this study, the ability of V2O3 to induce chromosomal damage and impair cell proliferation was tested on human leukocytes in vitro. The cultures cells were treated for 48 h with different concentrations 2, 4, 8 or 16 μg/mL of V2O3, and we use the sister chromatid exchange’s (SCE) test and the viability assay to evaluate the effects.In the results, no change was observed in either the viability or the frequency of SCE; however, a significant increase was observed in the incidence of premature chromatid separation (PCS), and a decrease was observed in both the mitotic index (MI) and the replication index (RI). Therefore, it can be suggested that V2O3 induces a genoto...
Databáze: OpenAIRE
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