In Vitro Evaluation of Antiproliferative Properties of Novel Organotin(IV) Carboxylate Compounds with Propanoic Acid Derivatives on a Panel of Human Cancer Cell Lines

Autor: Bojan Đ. Božić, Goran N. Kaluđerović, Nebojša Banjac, Ludger A. Wessjohann, Nebojša Pantelić, Bojana B. Zmejkovski, Biljana Dojčinović
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Cytotoxicity
Pharmaceutical Science
Organic chemistry
Apoptosis
triphenyltin(IV)
Ligands
01 natural sciences
Mass Spectrometry
Analytical Chemistry
chemistry.chemical_compound
Mice
QD241-441
Breast cancer
Neoplasms
Drug Discovery
Organotin Compounds
Caspase
biology
Chemistry
apoptosis
Triphenyltin(IV)
Reactive Nitrogen Species
3. Good health
Chemistry (miscellaneous)
Metals
MCF-7 Cells
Molecular Medicine
cytotoxicity
Female
medicine.symptom
HT29 Cells
Caspase 3
In Vitro Techniques
010402 general chemistry
Article
Inhibitory Concentration 50
breast cancer
Cell Line
Tumor
medicine
ICP-MS
Animals
Humans
Physical and Theoretical Chemistry
010405 organic chemistry
Molecular biology
In vitro
0104 chemical sciences
Propanoic acid
Mechanism of action
Cell culture
biology.protein
NIH 3T3 Cells
Gentian Violet
Drug Screening Assays
Antitumor
Reactive Oxygen Species
HeLa Cells
Zdroj: Molecules
Molecules, Vol 26, Iss 3199, p 3199 (2021)
Volume 26
Issue 11
ISSN: 1420-3049
Popis: The synthesis of novel triphenyltin(IV) compounds, Ph3SnLn (n = 1–3), with oxaprozin (3-(4,5-diphenyloxazol-2-yl)propanoic acid), HL1, and the new propanoic acid derivatives 3-(4,5-bis(4-methoxylphenyl)oxazol-2-yl)propanoic acid, HL2, and 3-(2,5-dioxo-4,4-diphenylimidazolidin-1-yl)propanoic acid, HL3, has been performed. The ligands represent commercial drugs or their derivatives and the tin complexes have been characterized by standard analytical methods. The in vitro antiproliferative activity of both ligands and organotin(IV) compounds has been evaluated on the following tumour cell lines: human prostate cancer (PC-3), human colorectal adenocarcinoma (HT-29), breast cancer (MCF-7), and hepatocellular cancer (HepG2), as well as on normal mouse embryonic fibroblast cells (NIH3T3) with the aid of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-12 diphenyltetrazolium bromide) and CV (crystal violet) assays. Contrary to the inactive ligand precursors, all organotin(IV) carboxylates showed very good activity with IC50 values ranging from 0.100 to 0.758 µM. According to the CV assay (IC50 = 0.218 ± 0.025 µM), complex Ph3SnL1 demonstrated the highest cytotoxicity against the caspase 3 deficient MCF-7 cell line. Inductively coupled plasma mass spectrometry (ICP-MS) analysis indicated a two-fold lower concentration of tin in MCF-7 cells in comparison to platinum. To investigate the mechanism of action of the compound Ph3SnL1 on MCF-7 cells, morphological, autophagy and cell cycle analysis, as well as the activation of caspase and ROS/RNS and NO production, has been performed. Results suggest that Ph3SnL1 induces caspase-independent apoptosis in MCF-7 cells.
Databáze: OpenAIRE
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