Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk
| Autor: | Chantal Brees, Ye Shih Ho, Marc Fransen, Stanley R. Terlecky, Oksana Ivashchenko, Paul P. Van Veldhoven |
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| Rok vydání: | 2011 |
| Předmět: |
Saccharomyces cerevisiae
Mitochondrion medicine.disease_cause Redox Cell Line Mice Chlorocebus aethiops Peroxisomes medicine Animals Humans Molecular Biology Cellular Senescence Organelles chemistry.chemical_classification Reactive oxygen species biology Articles Cell Biology Peroxisome Catalase Cell biology Mice Inbred C57BL Oxidative Stress chemistry Cell Biology of Disease COS Cells biology.protein Signal transduction Reactive Oxygen Species Oxidation-Reduction Cell aging Oxidative stress Signal Transduction |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Peroxisomes are capable of reactive oxygen species (ROS) generation, but their contribution to cellular redox balance is not well understood. This study demonstrates that peroxisomes and mitochondria functionally interact via ROS signaling, suggesting a potential broader role for the peroxisome in cellular aging and the initiation and progression of age-related diseases. Reactive oxygen species (ROS) are at once unsought by-products of metabolism and critical regulators of multiple intracellular signaling cascades. In nonphotosynthetic eukaryotic cells, mitochondria are well-investigated major sites of ROS generation and related signal initiation. Peroxisomes are also capable of ROS generation, but their contribution to cellular oxidation–reduction (redox) balance and signaling events are far less well understood. In this study, we use a redox-sensitive variant of enhanced green fluorescent protein (roGFP2-PTS1) to monitor the state of the peroxisomal matrix in mammalian cells. We show that intraperoxisomal redox status is strongly influenced by environmental growth conditions. Furthermore, disturbances in peroxisomal redox balance, although not necessarily correlated with the age of the organelle, may trigger its degradation. We also demonstrate that the mitochondrial redox balance is perturbed in catalase-deficient cells and upon generation of excess ROS inside peroxisomes. Peroxisomes are found to resist oxidative stress generated elsewhere in the cell but are affected when the burden originates within the organelle. These results suggest a potential broader role for the peroxisome in cellular aging and the initiation of age-related degenerative disease. |
| Databáze: | OpenAIRE |
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