Recombinant parvovirus B19 capsids as a new substrate for detection of B19-specific IgG and IgM antibodies by an enzyme-linked immunosorbent assay
| Autor: | Willy J. M. Spaan, Mario J.A.W.M. van Bussel, M. M. M. Salimans, Caroline S. Brown |
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| Rok vydání: | 1992 |
| Předmět: |
viruses
Erythema Infectiosum Enzyme-Linked Immunosorbent Assay Immunofluorescence Antibodies Viral Virus law.invention Substrate Specificity Capsid Antigen law hemic and lymphatic diseases Virology medicine Parvovirus B19 Human Humans biology medicine.diagnostic_test Parvovirus virus diseases Radioimmunoassay biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Recombinant Proteins Immunoglobulin M Immunoglobulin G biology.protein Recombinant DNA Antibody |
| Zdroj: | Journal of virological methods. 39(3) |
| ISSN: | 0166-0934 |
| Popis: | A new enzyme-linked immunosorbent assay for the detection of B19-specific IgG and IgM antibodies was established using B19 capsids synthesized in a baculovirus expression system. These B19 capsids, consisting of either coat protein VP2 alone or of both VP1 and VP2, have been shown to be similar to native virus in size and appearance. The results obtained for the detection of B19-specific antibodies showed good correlations with a radioimmunoassay which uses native B19 virus and an immunofluorescence assay based on insect cells expressing coat protein VP1. The course of the antibody response could be followed by determining the titers of sequential serum samples taken after a recent B19 infection. Both types of recombinant capsids form an excellent source of antigen for the detection of both B19 IgG and IgM antibodies and are a very promising substitute for native virus. |
| Databáze: | OpenAIRE |
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