Simulated-Use Polytetrafluorethylene Biofilm Model: Repeated Rounds of Complete Reprocessing Lead to Accumulation of Organic Debris and Viable Bacteria
| Autor: | Carol Reidy, Harminder Singh, Donald R. Duerksen, Nancy Olson, Zoann Nugent, Michelle J. Alfa, Gale Schultz, Pat DeGagne |
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| Rok vydání: | 2017 |
| Předmět: |
Microbiology (medical)
Epidemiology medicine.disease_cause Enterococcus faecalis Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Peracetic acid medicine 030212 general & internal medicine Polytetrafluoroethylene ENZYMATIC CLEANER Chromatography biology Pseudomonas aeruginosa Biofilm Biofilm matrix Bacterial persistence biology.organism_classification Disinfection Infectious Diseases chemistry Biofilms Microscopy Electron Scanning Equipment Contamination 030211 gastroenterology & hepatology Bacteria |
| Zdroj: | Infection Control & Hospital Epidemiology. 38:1284-1290 |
| ISSN: | 1559-6834 0899-823X |
| DOI: | 10.1017/ice.2017.215 |
| Popis: | OBJECTIVEBiofilm has been implicated in bacterial persistence and survival after endoscope reprocessing. In this study, we assessed the impact of different methods of reprocessing on organic residues and viable bacteria after repeated rounds of biofilm formation when each was followed by full reprocessing.METHODSATS-2015, an artificial test soil containing 5–8 Log10 colony-forming units (CFU) of Enterococcus faecalis and Pseudomonas aeruginosa, was used to form biofilm in polytetrafluroethylene channels overnight on 5 successive days. Each successive day, full pump-assisted cleaning using bristle brushes or pull-through devices in combination with enzymatic or nonenzymatic detergents followed by fully automated endoscope reprocessor disinfection using peracetic acid was performed. Residuals were visualized by scanning electron microscopy (SEM). Destructive testing was used to assess expected cutoffs for adenosine triphosphate (ATP; 2), and viable bacteria count (0 CFU).RESULTSProtein residuals were above 2 µg/cm2, but ATP residuals were CONCLUSIONSSurviving E. faecalis and P. aeruginosa were only detected when the non-enzymatic detergent was used, emphasizing the importance of the detergent used for endoscope channel reprocessing. Preventing biofilm formation is critical because not all current reprocessing methods can reliably eliminate viable bacteria within the biofilm matrix.Infect Control Hosp Epidemiol 2017;38:1284–1290 |
| Databáze: | OpenAIRE |
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