Additional file 2: Figure S2. of The functional divergence between SPA1 and SPA2 in Arabidopsis photomorphogenesis maps primarily to the respective N-terminal kinase-like domain

Autor: Chen, Song, Wirthmueller, Lennart, Stauber, Johannes, Lory, Niels, Holtkotte, Xu, Leson, Lisa, Schenkel, Christian, Ahmad, Margaret, Hoecker, Ute
Rok vydání: 2016
DOI: 10.6084/m9.figshare.c.3622013_d3.v1
Popis: The N-terminal domain and the WD-repeat domain of SPA2 render the chimeric SPA1 proteins more unstable in light-grown seedlings. A-C. SPA-HA protein levels in 4-day-old T2 DS_211-HA (A), DS_121-HA (B) and DS_112-HA (C) transgenic spa1 spa2 spa3 seedlings. Seedlings were grown in darkness (D) for 4 days and subsequently transferred to 0.35 μmol m−2 s−1 FR for 30 min. All transgenes were expressed under the control of the SPA2 promoter. SPA-HA was detected using an α–HA antibody. HSC70 levels served as a loading control. (PDF 349 kb)
Databáze: OpenAIRE