Retracted: Effects of astragalus polysaccharide on apoptosis of myocardial microvascular endothelial cells in rats undergoing hypoxia/reoxygenation by mediation of the PI3K/Akt/eNOS signaling pathway
| Autor: | Guowei Meng, Qingling Zhou, Fei Teng, Yongshan Zhang, Qiang Sun |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Nitric Oxide Synthase Type III Apoptosis Nitric Oxide Models Biological Biochemistry Rats Sprague-Dawley Phosphatidylinositol 3-Kinases 03 medical and health sciences Polysaccharides Enos Internal medicine medicine Animals Viability assay Phosphorylation Molecular Biology Protein kinase B Cells Cultured PI3K/AKT/mTOR pathway Cell Proliferation biology Chemistry Cell growth Myocardium Endothelial Cells Astragalus Plant Cell Biology biology.organism_classification Molecular biology Cell Hypoxia Rats Oxygen 030104 developmental biology Endocrinology Signal transduction Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Journal of Cellular Biochemistry. 119:806-816 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.26243 |
| Popis: | The study explores the effect of astragalus polysaccharide (APS) mediating P13K/Akt/eNOS signaling pathway on apoptosis of myocardial microvascular endothelial cells (MMECs) in hypoxia/reoxygenation (H/R). MMECs were classified into blank, H/R, H/R + 25 mg/L APS, H/R + 50 mg/L APS, H/R + 100 mg/L APS, H/R + LY, and HR + 100 mg/L APS + LY groups. Cell viability was detected using MTT assay and apoptotic cell morphological changes by Hoechst staining. NO content, cell cycle and apoptosis, PI3K/Akt/eNOS signaling pathway proteins were detected using nitrate reductase assay, flow cytometry and Western blotting. An increased cell survival rate, NO content and expression of PI3K/Akt/eNOS signaling pathway associated proteins, and a decreased apoptosis rate was observed in the H/R + 50 mg/L APS and H/R + 100 mg/L APS groups compared with the H/R and H/R + 25 mg/L APS groups. Compared with the H/R + 50 mg/L APS group, the apoptosis rate decreased, whereas the cell survival rate, NO content and expression of PI3K/Akt/eNOS signaling pathway associated proteins increased in the H/R + 100 mg/L APS group. The H/R + LY and HR + 100 mg/L APS + LY groups followed opposite trends. In comparison to the HR + 100 mg/L APS group, the apoptosis rate in the H/R + LY and HR + 100 mg/L APS + LY groups increased, and the cell survival rate, NO content and expression of PI3K/Akt/eNOS signaling pathway associated proteins decreased. Collectively, APS improves the damage caused by H/P by mediating PI3K/Akt/eNOS signaling pathway. |
| Databáze: | OpenAIRE |
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