Protective Effects of Puerarin on Premature Ovarian Failure via Regulation of Wnt/β-catenin Signaling Pathway and Oxidative Stress
| Autor: | Cheng Chen, Weiwei Cao, Jia Li, Cong Hu, Jian Huang, Li-Ping Zheng, Qingfeng Fu, Song Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
SOD2 Apoptosis Primary Ovarian Insufficiency medicine.disease_cause Protective Agents Andrology 03 medical and health sciences Follicle chemistry.chemical_compound Mice 0302 clinical medicine Cyclin D1 Ovarian Follicle Puerarin medicine Animals Busulfan Cyclophosphamide Wnt Signaling Pathway 030219 obstetrics & reproductive medicine business.industry Wnt signaling pathway Obstetrics and Gynecology medicine.disease Isoflavones Premature ovarian failure Disease Models Animal Oxidative Stress 030104 developmental biology chemistry Female Folliculogenesis business Oxidative stress |
| Zdroj: | Reproductive sciences (Thousand Oaks, Calif.). 28(4) |
| ISSN: | 1933-7205 |
| Popis: | This study was designed to investigate the protective effects of puerarin (PUE), which work via the Wnt/β-catenin signaling pathway, and oxidative stress in the premature ovarian failure (POF) model. Two-month-old female mice were randomly divided into four groups. One group was used as the control, and the other three groups were injected with cyclophosphamide and busulfan to create POF models. Two POF treatment groups were gavaged with 100 or 200 mg/kg PUE for 28 days. Next, the ovaries were fixed, and the numbers of different stage follicles were measured, and the ovarian surface epithelium (OSE) was collected. Oct4 and Mvh expression, Wnt/β-catenin signaling pathway activity, the oxidative stress factors SOD2 and Nrf2, and the apoptosis-related proteins Bcl-2 and Bax were detected by IHC, RT-QPCR, and western blotting. We found that the number of follicles, Oct4 and Mvh expression, and Wnt/β-catenin-signaling activity were reduced in the POF groups (p |
| Databáze: | OpenAIRE |
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