GLC analysis of metapyrilene in plasma and urine using a sulfur-specific flame-photometric detector
| Autor: | R.E. Schirmer, R.J. Pierson |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Chloroform
Chromatography Chromatography Gas Pyridines Metabolite Methapyrilene Cyclohexanedimethanol Pharmaceutical Science chemistry.chemical_element Urine Thiophenes Hydrogen-Ion Concentration Ethylenediamines Sulfur Photometry chemistry.chemical_compound chemistry medicine Histamine H1 Antagonists Methods Methanol Methyl silicone medicine.drug |
| Zdroj: | Journal of pharmaceutical sciences. 62(12) |
| ISSN: | 0022-3549 |
| Popis: | A GLC procedure was developed for analysis of methapyrilene in blood and urine. Samples are extracted with chloroform, dried, dissolved in methanol, and injected on a 1.22- m. (4-ft.) glass column packed with 1% cyclohexanedimethanol succinate and 10% of a 5 %-substituted phenyl methyl silicone on acid-washed, silylated diatomaceous earth and operated at 220°. A sulfur-specific flame-photometric detector is used, which allows concentrations as low as 50 ng./ml. to be measured. Methapyrilene was not detected in plasma or urine of human subjects receiving normal clinical doses of methapyrilene hydrochloride, but a methapyrilene metabolite was found in the urine samples. The absence of detectable levels of methapyrilene is probably due to rapid conversion of this compound to a metabolite that is not detected by the GLC procedure. |
| Databáze: | OpenAIRE |
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