Isolation of Naegleria spp. from a Brazilian Water Source
| Autor: | Otavio Henrique Thiemann, Ana Letícia Moreira da Fonseca, María Reyes-Batlle, Natália Karla Bellini, Odete Rocha, Jacob Lorenzo-Morales |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
food.ingredient Water source Biodiversity Zoology lcsh:Medicine free-living amoeba biology_other Naegleria Article Amoeba (genus) Monjolinho River food parasitic diseases Immunology and Allergy Internal transcribed spacer Molecular Biology Naegleria fowleri General Immunology and Microbiology biology Naegleria spp lcsh:R Ribosomal RNA biology.organism_classification AMOEBA Infectious Diseases PCR Hartmannella Brazil |
| Zdroj: | Pathogens Volume 9 Issue 2 Pathogens, Vol 9, Iss 2, p 90 (2020) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens9020090 |
| Popis: | The genus Naegleria, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the &ldquo brain-eating amoeba,&rdquo is the most studied Naegleria species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying Naegleria spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of Sã o Carlos, in Sã o Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni sequences, as well as a Hartmannella sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil. |
| Databáze: | OpenAIRE |
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