Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification
| Autor: | David Parma, Robert E. Boswell, Paul E. Bennett |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Cellular differentiation
Genes Insect RNA/protein localization Germline Article Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Oogenesis medicine Animals Drosophila Proteins Molecular Biology 030304 developmental biology Genetics 0303 health sciences biology Gene Expression Regulation Developmental Nuclear Proteins RNA-Binding Proteins Cell Differentiation Cell Biology Oocyte biology.organism_classification Null allele Exon junction complex medicine.anatomical_structure Drosophila melanogaster Germ Cells Centrosome Drosophila oogenesis Oocytes Female Stem cell 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. 308(2):507-519 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2007.06.007 |
| Popis: | A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprotein complex assembled on spliced mRNAs. In previous studies, reduced function alleles of mago nashi and tsunagi/Y14 were used to characterize the roles of the genes in oogenesis. Here, we investigated mago nashi and tsunagi/Y14 using null alleles and clonal analysis. Germline clones lacking mago nashi function divide but fail to differentiate. The mago nashi null germline stem cells produce clones over a period of at least 11 days, suggesting that mago nashi is not necessary for stem cell self-renewal. However, germline stem cells lacking tsunagi/Y14 function are indistinguishable from wild type. Additionally, in tsunagi/Y14 null germline cysts, centrosomes and oocyte-specific components fail to concentrate within a single cell and oocyte fate is not restricted to a single cell. Together, our results suggest not only that mago nashi is required for germline stem cell differentiation but that surprisingly mago nashi functions independently of tsunagi/Y14 in this process. On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process. |
| Databáze: | OpenAIRE |
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