Expression and regulation of β7(βp) integrins on mouse lymphocytes: Relevance to the mucosal immune system
| Autor: | Peter J. Kilshaw, Susan J. Murant |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Integrins
Macromolecular Substances T-Lymphocytes Blotting Western Immunology Integrin Mice Peyer's Patches Antibody Specificity Transforming Growth Factor beta Animals Immunology and Allergy Intestinal Mucosa biology Antibodies Monoclonal T lymphocyte Flow Cytometry Precipitin Tests Molecular biology Gut-specific homing Integrin alpha M biology.protein Intraepithelial lymphocyte Lymph Nodes Antibody Alpha chain ATP synthase alpha/beta subunits |
| Zdroj: | European Journal of Immunology. 21:2591-2597 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830211041 |
| Popis: | A mouse lymphocyte surface molecule which is selectively expressed by mucosal T cells and detected with the monoclonal antibody (mAb) M290 has provisionally been identified as a beta 7 integrin. Identification was based on close homology of its beta subunit at the N terminus with the recently reported, highly distinctive, human beta 7 sequence. mAb were prepared against the beta and alpha subunits of the mouse molecule, termed beta 7 and alpha M290, respectively, and used to study surface expression of the two components. beta 7 was present on most lymph node lymphocytes in association with alpha 4 rather than alpha M290. This integrin, beta 7 alpha 4, was shown to be identical to LPAM-1 (beta p alpha 4) the Peyer's patch homing receptor. Stimulation in vitro of mouse lymph node T cells with anti-CD3 in the presence of transforming growth factor (TGF)-beta increased beta 7 expression in about 40% of cells and changed the associated alpha chain from alpha 4 to the novel alpha M290 subunit, which, in most cells, was expressed de novo. Immunoprecipitation of beta 7 both from these cells and from intraepithelial lymphocytes gave closely similar results and showed predominance of the beta 7 alpha M290 integrin. It is suggested that in vivo this change in alpha-chain usage occurs in mucosal T cells in response to TGF-beta acting in the mucosal microenvironment and that the new integrin confers particular adhesive properties, possibly homing specificity, on the cells. |
| Databáze: | OpenAIRE |
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