Characterisation and microbial community analysis of lipid utilising microorganisms for biogas formation

Autor:	Marwan Al-Momani, Jae-Ho Shin, Gi-Ung Kang, Mazen K. Nazal, Shaikh A. Razzak, Alexis Nzila, Saravanan Sankara, Jerald Conrad Ibal
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0106 biological sciences Glycerol Salinity Methanogens Chemical Oxygen Demand Porphyromonadaceae Biogas 01 natural sciences Biochemistry Physical Chemistry Bioreactors Water Quality Bioenergy Food science Materials Biotransformation 0303 health sciences education.field_of_study Carbon Monoxide Multidisciplinary biology Chemistry Microbiota Monomers Fatty Acids Lipids Physical Sciences Medicine Engineering and Technology Proteobacteria Research Article Anaerobic respiration Methanogenesis Firmicutes Science Population Materials Science Fuels 03 medical and health sciences Oxygen Consumption 010608 biotechnology education Olive Oil 030304 developmental biology Ecology and Environmental Sciences Organisms Bacteroidetes Biology and Life Sciences biology.organism_classification Polymer Chemistry Lipid Metabolism Archaea Energy and Power Kinetics Microbial population biology Chemical Properties Biofuels Oils Oleic Acid
Zdroj:	PLoS ONE PLoS ONE, Vol 14, Iss 11, p e0224989 (2019)
ISSN:	1932-6203
Popis:	In the anaerobic process, fat-oil-grease (FOG) is hydrolysed to long-chain fatty acids (LCFAs) and glycerol (GLYC), which are then used as substrates to produce biogas. The increase in FOG and LCFAs inhibits methanogenesis, and so far, most work investigating this inhibition has been carried out when FOG or LCFAs were used as co-substrates. In the current work, the inhibition of methanogenesis by FOG, LCFAs and GLYC was investigated when used as sole substrates. To gain more insight on the dynamics of this process, the change of microbial community was analysed using 16S rRNA gene amplicon sequencing. The results indicate that, as the concentrations of cooking olive oil (CO, which represents FOG) and LCFAs increase, methanogenesis is inhibited. For instance, at 0.01 g. L-1 of FOG, the rate of biogas formation was around 8 ml.L-1.day-1, and this decreased to = 45°C and NaCl > 3% led to a significant decrease in the rate of biogas formation. Microbial community analyses were carried out from samples from 3 different bioreactors (CO, OLEI and GLYC), on day 1, 5 and 15. In each bioreactor, microbial communities were dominated by Proteobacteria, Firmicutes and Bacteroidetes phyla. The most important families were Enterobacteriaceae, Pseudomonadaceae and Shewanellaceae (Proteobacteria phylum), Clostridiacea and Ruminococcaceae (Firmicutes) and Porphyromonadaceae and Bacteroidaceae (Bacteroidetes). In CO bioreactor, Proteobacteria bacteria decreased over time, while those of OLEI and GLYC bioreactors increased. A more pronounced increase in Bacteroidetes and Firmicutes were observed in CO bioreactor. The methanogenic archaea Methanobacteriaceae and Methanocorpusculaceae were identified. This analysis has shown that a set of microbial population is selected as a function of the substrate.
Databáze:	OpenAIRE
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