Specific In Planta Recognition of Two GKLR Proteins of the Downy Mildew Bremia lactucae Revealed in a Large Effector Screen in Lettuce

Autor: Joost H. M. Stassen, Erik den Boer, Guido Van den Ackerveken, Johan W. Schut, Annemiek Andel, Pim W. J. Vergeer, Ursula Ellendorff, Mathieu A. Pel, Marieke J. W. Jeuken, Olaf Zonneveld, K.T.B. Pelgrom
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Physiology
Amino Acid Motifs
Lactuca
Breeding
Bremia
Laboratorium voor Plantenveredeling
wild lettuce
avirulence genes
Plant Proteins
Genetics
Bremia lactucae
Effector
Chromosome Mapping
General Medicine
genetic-linkage map
Lettuce
saligna
Protein Transport
Phenotype
Oomycetes
Multigene Family
Host-Pathogen Interactions
potato
Hypersensitive response
disease resistance
phytophthora-infestans
Molecular Sequence Data
Locus (genetics)
Biology
Plant disease resistance
Protein Sorting Signals
Chromosomes
Plant
Botany
nonhost resistance
Amino Acid Sequence
Alleles
Plant Diseases
backcross inbred lines
Proteins
biology.organism_classification
Laboratorium voor Phytopathologie
Plant Leaves
Plant Breeding
Gene Expression Regulation
Laboratory of Phytopathology
Downy mildew
EPS
Agronomy and Crop Science
Sequence Alignment
pathogen
Zdroj: Molecular Plant-Microbe Interactions 26 (2013) 11
Molecular Plant-Microbe Interactions, 26(11), 1259-1270
ISSN: 0894-0282
1259-1270
Popis: Breeding lettuce (Lactuca sativa) for resistance to the downy mildew pathogen Bremia lactucae is mainly achieved by introgression of dominant downy mildew resistance (Dm) genes. New Bremia races quickly render Dm genes ineffective, possibly by mutation of recognized host-translocated effectors or by suppression of effector-triggered immunity. We have previously identified 34 potential RXLR(-like) effector proteins of B. lactucae that were here tested for specific recognition within a collection of 129 B. lactucae-resistant Lactuca lines. Two effectors triggered a hypersensitive response: BLG01 in 52 lines, predominantly L. saligna, and BLG03 in two L. sativa lines containing Dm2 resistance. The N-terminal sequences of BLG01 and BLG03, containing the signal peptide and GKLR variant of the RXLR translocation motif, are not required for in planta recognition but function in effector delivery. The locus responsible for BLG01 recognition maps to the bottom of lettuce chromosome 9, whereas recognition of BLG03 maps in the RGC2 cluster on chromosome 2. Lactuca lines that recognize the BLG effectors are not resistant to Bremia isolate Bl:24 that expresses both BLG genes, suggesting that Bl:24 can suppress the triggered immune responses. In contrast, lettuce segregants displaying Dm2-mediated resistance to Bremia isolate Bl:5 are responsive to BLG03, suggesting that BLG03 is a candidate Avr2 protein.
Databáze: OpenAIRE
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