Gene-specific promoter methylation is associated with micronuclei frequency in urothelial cells from individuals exposed to organic solvents and paints
| Autor: | Diana Saavedra-Trujillo, M Ruíz, Ingrid Reyes-Carvajal, A Tello, Silvio Carvajal, Luisa F. Escobar-Hoyos, A Muñoz, Luz Stella Hoyos-Giraldo, Regina M. Santella, Elizabeth Londoño-Velasco, Nohelia Cajas-Salazar |
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| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Epidemiology Biology Toxicology CDH1 03 medical and health sciences GSTP1 0302 clinical medicine Occupational Exposure Paint Humans Promoter Regions Genetic neoplasms Gene Micronucleus Tests Public Health Environmental and Occupational Health Promoter DNA Methylation Pollution Molecular biology 030104 developmental biology 030220 oncology & carcinogenesis DNA methylation Immunology Micronucleus test Solvents biology.protein Urothelium Chromosome breakage Micronucleus |
| Zdroj: | Journal of Exposure Science & Environmental Epidemiology. 26:257-262 |
| ISSN: | 1559-064X 1559-0631 |
| DOI: | 10.1038/jes.2015.28 |
| Popis: | Sufficient epidemiologic evidence has established an etiologic link between bladder cancer risk and occupational exposure as a painter to organic solvents. Currently, it remains to be established whether gene-specific promoter methylation contributes to bladder cancer development, including by enhancing chromosome breakage or loss. We investigated the effect of chronic exposure to organic solvents and paints on DNA methylation profiles in the promoter regions of four genes (GSTP1, p16(INK4a), APC and CDH1) and micronucleus (MN) frequency in exfoliated urothelial cells from voided urine from Colombian male non-smoking car painters and age-matched unexposed individuals. The exposed group had a higher percentage of individuals with2 MNs/2000 cells compared with the unexposed group (P=0.04). Gene-specific analysis showed a significantly higher percentage of individuals with methylated GSTP1, p16(INK4a) and APC in the exposed group. Poisson regression analysis indicated that exposed individuals with methylated GSTP1 and p16(INK4a) promoters were more than twofold more likely to have an increase in MN frequency as compared with the reference. Finally, among exposed individuals with GSTP1 and p16(INK4a) methylated promoters, those with a greater age had a higher RR of increased MN frequency compared with younger exposed individuals with methylated promoters. These results support the conclusion that gene-specific promoter methylation may increase MN frequency in a dependent or independent interaction with occupational exposure to organic solvents. |
| Databáze: | OpenAIRE |
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