Analysis of adult otitis media: polymerase chain reaction versus culture for bacteria and viruses
| Autor: | G. J. White, J C Post, E M Liederman, Garth D. Ehrlich, D A Sirko, Jerome J. Aul, Craig A. Buchman |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Virus Cultivation medicine.disease_cause Polymerase Chain Reaction Virus Statistics Nonparametric law.invention Haemophilus influenzae Adenoviridae Moraxella catarrhalis 03 medical and health sciences 0302 clinical medicine law Streptococcus pneumoniae medicine Humans 030223 otorhinolaryngology Polymerase chain reaction Aged Aged 80 and over Bacteriological Techniques biology Viral culture Otitis Media with Effusion Nucleic Acid Hybridization General Medicine Middle Aged biology.organism_classification Virology Otitis Otorhinolaryngology 030220 oncology & carcinogenesis HIV-1 Female Viral disease medicine.symptom Oligonucleotide Probes |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0003-4894 |
| Popis: | Recent studies using the polymerase chain reaction (PCR) have identified bacterial and viral genomic sequences in culture-negative pediatric middle ear effusions. To evaluate this technique in adults, 19 effusions were analyzed to compare bacterial and viral culture and PCR detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and adenovirus. Effusions from 4 subjects positive for human immunodeficiency virus (HIV) were analyzed by PCR for HIV virus. Three of 19 effusions were culture-positive for bacteria, and 0 of 19 for viruses. Fifteen of 19 effusions were PCR-positive for bacterial genomic sequences, and 0 of 19 for adenovirus. Thirteen of 15 PCR-positive specimens demonstrated 5 pneumoniae, 5 of 15 H influenzae, and 0 of 13 M catarrhalis. All 4 effusions from HIV-positive subjects were PCR-positive for HIV. No effusion was culture-positive and PCR-negative. These results confirm that culture-negative middle ear effusions contain genomic sequences from bacterial pathogens. Finding of HIV RNA and DNA in effusion from HIV-positives suggests replicating virus in this fluid. |
| Databáze: | OpenAIRE |
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