Time is life: EpAssistant - a new tool for the automatic identification of anti-HLA antibody epitope specificity in transplant programs
| Autor: | Vinicius Ponte Machado, Adalberto Socorro da Silva, Renato S. Vieira, José Machado Moita Neto, Keylla Maria de Sá Urtiga Aita, Semiramis Jamil Hadad do Monte, Luiz Claudio Demes da Mata Sousa |
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| Rok vydání: | 2018 |
| Předmět: |
Graft Rejection
Computer science Immunology Anti-HLA antibody Human leukocyte antigen Computational biology 030230 surgery Epitope 03 medical and health sciences Epitopes 0302 clinical medicine Hla molecules HLA Antigens Isoantibodies Immunology and Allergy Humans Epitope specificity Transplantation Electronic Data Processing Histocompatibility Testing Kidney Transplantation Transplant Recipients Identification (information) Epitopes B-Lymphocyte Immunization Algorithms Software 030215 immunology |
| Zdroj: | Transplant immunology. 51 |
| ISSN: | 1878-5492 |
| Popis: | Background and objectives This work aims to present the expert system EpAssistant, a platform algorithm designed to accurately and automatically identify the EPLETS specificities of anti-HLA (Human Leukocyte Antigen) antibodies in the sera of transplantation candidates. Materials and methods The algorithm was written in the Python™ language and, at this time, integrated with the EpViX system ( www.epvix.com.br ). EpAssistant uses JSON parameters of the Single-Antigen Bead (SAB) assays from both vendors, the patient's HLA typing and the Cut Off (CO) value as inputs. The outputs of the tool are the EPLETSS explaining the reactivity patterns of anti-HLA antibodies in the patient's serum. Results As preliminary results, we present the development and establishment of the EpAssistant platform. EpAssistant analyses can be performed for Class I (−A, B and C) and Class II (−DR, −DQ and -DP) HLA molecules. Conclusions EpAssistant automates the EPLETS reactivity analysis process and drastically reduces the time required to produce final results, enabling large-scale data analyses in a simple, inexpensive and rapid manner and facilitating the allocation of donated organs via the EPLETS Virtual Crossmatch (EVxM) system. |
| Databáze: | OpenAIRE |
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