Uptake of microparticle-adsorbed protein antigen by bone marrow-derived dendritic cells results in up-regulation of interleukin-1α and interleukin-12 p40/p35 and triggers prolonged, efficient antigen presentation
| Autor: | Hans-Peter Dienes, Maria Mehlig, Konrad Reske, Christoph Scheicher |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Immunology Antigen presentation Bone Marrow Cells Mice Phagocytosis Bone Marrow Animals Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Cells Cultured Interleukin 3 Antigen Presentation Mice Inbred C3H MHC class II CD40 biology Follicular dendritic cells Histocompatibility Antigens Class II Dendritic Cells Dendritic cell Interleukin-12 Molecular biology Up-Regulation Cell biology biology.protein Interleukin-1 |
| Zdroj: | European Journal of Immunology. 25:1566-1572 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830250615 |
| Popis: | Dendritic cells synthesize and express major histocompatibility complex (MHC) class II peptide-binding elements constitutively and, therefore, belong to the category of professional antigen-presenting cells. Unlike other cells that show constitutive class II expression, such as B cells and certain T cell clones, dendritic cells possess the unique capacity to activate naive T cells. Using dendritic cells generated in vitro by culture of mouse bone marrow in the presence of low doses of recombinant mouse granulocyte/macrophage colony-stimulating factor, we found that discrete maturation stages of these cells can be distinguished which were correlated with defined functional capabilities. The striking observation was the presence of a progenitor dendritic cell expressing low levels of class II which, unlike its differentiated counterpart in vitro, possessed pronounced phagocytic activity. Adding protein antigen to dendritic cells in a particle-adsorbed form, as compared to a soluble form, we demonstrate that phagocytosis of the particle-adsorbed protein by progenitor dendritic cells involves an activation event. This is evidenced by the de novo synthesis of transcripts of interleukin-1 alpha and interleukin-12 p40/p35 as well as transcripts of MHC class II. Most importantly, an augmented and prolonged antigen-presentation capacity was observed when the antigen was given in particle-adsorbed instead of soluble form. These findings indicate that progenitor dendritic cells are functionally more flexible and potent than fully differentiated dendritic cells and that they play a crucial role in antigen presentation. It is suggested that these findings will open up new possibilities to devise strategies for vaccine development. |
| Databáze: | OpenAIRE |
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