CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma

Autor: Kosuke Takigawa, Koji Iihara, Noritoshi Shirouzu, Masako Hosoi, Masahiro Ohgidani, Nobutaka Mukae, Takahiro A. Kato, Yuhei Sangatsuda, Satoshi O. Suzuki, Nobuhiro Hata, Masahiro Mizoguchi, Hideomi Hamasaki, Shogo Inamine, Yutaka Fujioka, Yusuke Funakoshi, Noriaki Sagata, Shunya Tanaka, Ryusuke Hatae, Toru Iwaki
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
medicine.medical_treatment
Immunology
Antigens
Differentiation
Myelomonocytic
microglia
IMG
03 medical and health sciences
0302 clinical medicine
Immune system
Downregulation and upregulation
Antigens
CD
Monitoring
Immunologic
Glioma
glioma
medicine
Biomarkers
Tumor
Tumor Microenvironment
Immunology and Allergy
Humans
Receptors
Immunologic
surrogate biomarker
Cells
Cultured
induced microglia-like cells
Original Research
Membrane Glycoproteins
Microglia
business.industry
Brain Neoplasms
Calcium-Binding Proteins
Microfilament Proteins
computer.file_format
Immunotherapy
RC581-607
medicine.disease
Prognosis
Phenotype
030104 developmental biology
medicine.anatomical_structure
CD206
Cancer research
Biomarker (medicine)
Feasibility Studies
Female
Immunologic diseases. Allergy
business
computer
030217 neurology & neurosurgery
Zdroj: Frontiers in Immunology
Frontiers in Immunology, Vol 12 (2021)
ISSN: 1664-3224
Popis: Targeting the unique glioma immune microenvironment is a promising approach in developing breakthrough immunotherapy treatments. However, recent advances in immunotherapy, including the development of immune checkpoint inhibitors, have not improved the outcomes of patients with glioma. A way of monitoring biological activity of immune cells in neural tissues affected by glioma should be developed to address this lack of sensitivity to immunotherapy. Thus, in this study, we sought to examine the feasibility of non-invasive monitoring of glioma-associated microglia/macrophages (GAM) by utilizing our previously developed induced microglia-like (iMG) cells. Primary microglia (pMG) were isolated from surgically obtained brain tissues of 22 patients with neurological diseases. iMG cells were produced from monocytes extracted from the patients’ peripheral blood. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed a significant correlation of the expression levels of representative markers for M1 and M2 microglia phenotypes between pMG and the corresponding iMG cells in each patient (Spearman’s correlation coefficient = 0.5225, P <0.0001). Synchronous upregulation of CD206 expression levels was observed in most patients with glioma (6/9, 66.7%) and almost all patients with glioblastoma (4/5, 80%). Therefore, iMG cells can be used as a minimally invasive tool for monitoring the disease-related immunological state of GAM in various brain diseases, including glioma. CD206 upregulation detected in iMG cells can be used as a surrogate biomarker of glioma.
Databáze: OpenAIRE
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