CX3CR1+ macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium
| Autor: | JH Niess, K Radulovic, N Garbi, Christian U. Riedel, V Rossini, C Manta, Esther Heupel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Immunology Antimicrobial peptides CX3C Chemokine Receptor 1 Biology digestive system Article Microbiology Interleukin 22 Mice Immunity Citrobacter rodentium Animals Immunology and Allergy Lymphocytes Mice Knockout Diphtheria toxin Phagocytes Innate immune system Interleukins Macrophages Innate lymphoid cell Enterobacteriaceae Infections Interleukin Immunity Innate CD11c Antigen Disease Models Animal Gene Expression Regulation Female Receptors Chemokine |
| Zdroj: | Mucosal Immunology |
| ISSN: | 1933-0219 |
| DOI: | 10.1038/mi.2012.61 |
| Popis: | Innate immune cells, such as intestinal epithelial cells, dendritic cells (DCs), macrophages, granulocytes, and innate lymphoid cells provide a first line of defence to enteric pathogens. To study the role of CX(3)CR1(+) DCs and macrophages in host defence, we infected CX(3)CR1-GFP animals with Citrobacter rodentium. When transgenic CX(3)CR1-GFP animals are infected with the natural mouse pathogen C. rodentium, CX(3)CR1(-/-) animals showed a delayed clearance of C. rodentium as compared with (age- and sex-matched) wild-type B6 animals. The delayed clearance of C. rodentium is associated with reduced interleukin (IL)-22 expression. In C. rodentium-infected CX(3)CR1-GFP animals, IL-22 producing lymphoid-tissue inducer cells (LTi cells) were selectively reduced in the absence of CX(3)CR1. The reduced IL-22 expression correlates with decreased expression of the antimicrobial peptides RegIIIβ and RegIIIγ. The depletion of CX(3)CR1(+) cells by diphtheria toxin injection in CX(3)CR1-GFP × CD11c.DOG animals confirmed the role of CX(3)CR1(+) phagocytes in establishing IL-22 production, supporting the clearance of a C. rodentium infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|